Single proline substitutions of selected helices of the Bacillus thuringiensis Cry4B toxin affect inclusion solubility and larvicidal activity

• Main Authors: I. Sramala, P. Uawithya, U. Chanama, S. Leetachewa, C. Krittanai, G. Katzenmeier, S. Panyim, C. Angsuthanasombat
• 其他作者: Mahidol University
• 格式: Article
• 出版: 2018
• 主題: Biochemistry, Genetics and Molecular Biology
• 在線閱讀: https://repository.li.mahidol.ac.th/handle/123456789/25883

PCR-based mutagenesis was employed to investigate the role in toxicity of putative trans-membrane helices of the 130-kDa Cry4B mosquito-larvicidal delta-endotoxin produced by Bacillus thuringiensis subsp. israelensis. Mutant toxins with a single proline substitution in the central region of α5, α6 a...