Redesign of an Interhelical Loop of the Bacillus thuringiensis Cry4B delta-endotoxin for Proteolytic Cleavage

• Main Authors: Chartchai Krittanai, Panida Lungchukiet, Sarinthip Ruangwetdee, Tipparut Tuntitippawan, Sakol Panyim, Gerd Katzenmeier, Chanan Angsuthanasombat
• Other Authors: Mahidol University
• Format: Article
• Published: 2018
• Subjects: Biochemistry, Genetics and Molecular Biology
• Online Access: https://repository.li.mahidol.ac.th/handle/123456789/26471

The mosquito-larvicidal Cry4B protein from Bacillus thuringiensis subsp. israelensis was expressed in Escherichia coli. Upon activation by trypsin, the 130-kDa protoxin was processed into the 65-kDa active toxin containing two polypeptide fragments of ca. 47 and ca. 20 kDa. These two polypeptides ar...