Kinetic study of protein formation and digestion by quartz crystal microbalance

Quartz crystal microbalance technique was used to study kinetic of formation and digestion of bovine serum albumin (BSA) protein on polystyrene-co-maleic acids (PSMA) surface. The kinetic of formation and digestion of BSA was investigated by measuring the frequency shift and resistance shift as a fu...

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Main Authors: Tippavan Hongkacharn, Verawat Champreda, Toemsak Srikhirin, Thidarat Wangkam, Tanakorn Osotchan
Other Authors: Mahidol University
Format: Conference or Workshop Item
Published: 2018
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Online Access:https://repository.li.mahidol.ac.th/handle/123456789/29090
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spelling th-mahidol.290902018-09-24T16:00:42Z Kinetic study of protein formation and digestion by quartz crystal microbalance Tippavan Hongkacharn Verawat Champreda Toemsak Srikhirin Thidarat Wangkam Tanakorn Osotchan Mahidol University Thailand National Science and Technology Development Agency Engineering Quartz crystal microbalance technique was used to study kinetic of formation and digestion of bovine serum albumin (BSA) protein on polystyrene-co-maleic acids (PSMA) surface. The kinetic of formation and digestion of BSA was investigated by measuring the frequency shift and resistance shift as a function of time. In order to study pH and concentration effect to layer formation kinetic, the pH of solution was varied from 2.0 to 7.4 while the concentration of BSA was varied in the range of 0.001 to 10 mg/ml. The kinetic of formation appears to be sensitive to the pH of solution and concentration. The formation layer at about pH 3.0 to 3.5 gives the different characteristic from the others. The layer formation increases as the concentration increase then reached the saturate value at the concentration of over 3 mg/ml. The kinetic of digestion was evaluate by applying proteinase enzyme on varies densities of BSA layer. It found that the rate of digestion depends on the density of the molecular BSA packing, modified by varying pH and concentration of BSA. ©2010 IEEE. 2018-09-24T09:00:42Z 2018-09-24T09:00:42Z 2010-05-05 Conference Paper INEC 2010 - 2010 3rd International Nanoelectronics Conference, Proceedings. (2010), 338-339 10.1109/INEC.2010.5424730 2-s2.0-77951655691 https://repository.li.mahidol.ac.th/handle/123456789/29090 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=77951655691&origin=inward
institution Mahidol University
building Mahidol University Library
continent Asia
country Thailand
Thailand
content_provider Mahidol University Library
collection Mahidol University Institutional Repository
topic Engineering
spellingShingle Engineering
Tippavan Hongkacharn
Verawat Champreda
Toemsak Srikhirin
Thidarat Wangkam
Tanakorn Osotchan
Kinetic study of protein formation and digestion by quartz crystal microbalance
description Quartz crystal microbalance technique was used to study kinetic of formation and digestion of bovine serum albumin (BSA) protein on polystyrene-co-maleic acids (PSMA) surface. The kinetic of formation and digestion of BSA was investigated by measuring the frequency shift and resistance shift as a function of time. In order to study pH and concentration effect to layer formation kinetic, the pH of solution was varied from 2.0 to 7.4 while the concentration of BSA was varied in the range of 0.001 to 10 mg/ml. The kinetic of formation appears to be sensitive to the pH of solution and concentration. The formation layer at about pH 3.0 to 3.5 gives the different characteristic from the others. The layer formation increases as the concentration increase then reached the saturate value at the concentration of over 3 mg/ml. The kinetic of digestion was evaluate by applying proteinase enzyme on varies densities of BSA layer. It found that the rate of digestion depends on the density of the molecular BSA packing, modified by varying pH and concentration of BSA. ©2010 IEEE.
author2 Mahidol University
author_facet Mahidol University
Tippavan Hongkacharn
Verawat Champreda
Toemsak Srikhirin
Thidarat Wangkam
Tanakorn Osotchan
format Conference or Workshop Item
author Tippavan Hongkacharn
Verawat Champreda
Toemsak Srikhirin
Thidarat Wangkam
Tanakorn Osotchan
author_sort Tippavan Hongkacharn
title Kinetic study of protein formation and digestion by quartz crystal microbalance
title_short Kinetic study of protein formation and digestion by quartz crystal microbalance
title_full Kinetic study of protein formation and digestion by quartz crystal microbalance
title_fullStr Kinetic study of protein formation and digestion by quartz crystal microbalance
title_full_unstemmed Kinetic study of protein formation and digestion by quartz crystal microbalance
title_sort kinetic study of protein formation and digestion by quartz crystal microbalance
publishDate 2018
url https://repository.li.mahidol.ac.th/handle/123456789/29090
_version_ 1763497575795654656