Acid‐extracted nuclear proteins and ultrastructure of human sperm chromatin as revealed by differential extraction with urea, mercaptoethanol, and salt
Basic proteins were differentially extracted from the purified heads of ejaculated human sperm by successive treatment with (1) 1% Triton X‐100, 1% mercaptoethanol (ME); (2) 8 M urea, 1% ME; (3) 8 M urea, 1% ME, 0.2 M NaCl; and (4) 8 M urea, 1% ME, 0.6 M NaCl. Nonhistones were extracted in the first...
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| Main Authors: | , , , |
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| Format: | Article |
| Published: |
2018
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| Online Access: | https://repository.li.mahidol.ac.th/handle/123456789/30148 |
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| Institution: | Mahidol University |