Peptide-based delivery of oligonucleotides across blood-brain barrier model
Delivery of pharmaceutical agents across a blood-brain barrier (BBB) is a challenge for brain cancer therapy. In this study, an in vitro BBB model was utilized to study the delivery of oligonucleotides across brain endothelial cells targeting to glioma cells in a Transwell™ setup. A series of novel...
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th-mahidol.334582018-11-09T10:12:34Z Peptide-based delivery of oligonucleotides across blood-brain barrier model Artita Srimanee Jakob Regberg Mattias Hallbrink Kaido Kurrikoff Kadi Liis Veiman Opa Vajragupta Ülo Langel Mahidol University Stockholms universitet University of Tartu Biochemistry, Genetics and Molecular Biology Chemical Engineering Chemistry Pharmacology, Toxicology and Pharmaceutics Delivery of pharmaceutical agents across a blood-brain barrier (BBB) is a challenge for brain cancer therapy. In this study, an in vitro BBB model was utilized to study the delivery of oligonucleotides across brain endothelial cells targeting to glioma cells in a Transwell™ setup. A series of novel peptides were synthesized by covalent conjugation of cell-penetrating peptides with targeting peptides for delivery of gene-based therapeutics. These peptides were screened for passage across the Transwell™ and we found the most efficient peptide PepFect32 from originating PepFect 14 coupled with the targeting peptide angiopep-2. PepFect32/pDNA nanocomplexes exhibited high transcytosis across the BBB in vitro model and the highest transfection efficiency to glioma cells. In conclusion, PepFect32 revealed the most efficient peptide-based vector for pDNA delivery across in vitro BBB model. © 2013 Springer Science+Business Media. 2018-11-09T01:59:22Z 2018-11-09T01:59:22Z 2014-01-01 Article International Journal of Peptide Research and Therapeutics. Vol.20, No.2 (2014), 169-178 10.1007/s10989-013-9378-4 15733904 15733149 2-s2.0-84899483274 https://repository.li.mahidol.ac.th/handle/123456789/33458 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84899483274&origin=inward |
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Biochemistry, Genetics and Molecular Biology Chemical Engineering Chemistry Pharmacology, Toxicology and Pharmaceutics Artita Srimanee Jakob Regberg Mattias Hallbrink Kaido Kurrikoff Kadi Liis Veiman Opa Vajragupta Ülo Langel Peptide-based delivery of oligonucleotides across blood-brain barrier model |
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Delivery of pharmaceutical agents across a blood-brain barrier (BBB) is a challenge for brain cancer therapy. In this study, an in vitro BBB model was utilized to study the delivery of oligonucleotides across brain endothelial cells targeting to glioma cells in a Transwell™ setup. A series of novel peptides were synthesized by covalent conjugation of cell-penetrating peptides with targeting peptides for delivery of gene-based therapeutics. These peptides were screened for passage across the Transwell™ and we found the most efficient peptide PepFect32 from originating PepFect 14 coupled with the targeting peptide angiopep-2. PepFect32/pDNA nanocomplexes exhibited high transcytosis across the BBB in vitro model and the highest transfection efficiency to glioma cells. In conclusion, PepFect32 revealed the most efficient peptide-based vector for pDNA delivery across in vitro BBB model. © 2013 Springer Science+Business Media. |
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Mahidol University |
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Mahidol University Artita Srimanee Jakob Regberg Mattias Hallbrink Kaido Kurrikoff Kadi Liis Veiman Opa Vajragupta Ülo Langel |
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Article |
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Artita Srimanee Jakob Regberg Mattias Hallbrink Kaido Kurrikoff Kadi Liis Veiman Opa Vajragupta Ülo Langel |
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Artita Srimanee |
title |
Peptide-based delivery of oligonucleotides across blood-brain barrier model |
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Peptide-based delivery of oligonucleotides across blood-brain barrier model |
title_full |
Peptide-based delivery of oligonucleotides across blood-brain barrier model |
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Peptide-based delivery of oligonucleotides across blood-brain barrier model |
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Peptide-based delivery of oligonucleotides across blood-brain barrier model |
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peptide-based delivery of oligonucleotides across blood-brain barrier model |
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2018 |
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https://repository.li.mahidol.ac.th/handle/123456789/33458 |
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1763488046039171072 |