An experiment illustrating the change in ligand pKa upon protein binding

• Main Authors: Pirom Chenprakhon, ภิรมย์ เชนประโคน, Bhinyo Panijpan, Pimchai Chaiyen
• Other Authors: Mahidol University. Institute for Innovative Learning
• Format: Article
• Language: English
• Published: 2015
• Subjects: Graduate education/research; Upper-division undergraduate; Analytical chemistry; Biochemistry; Laboratory instruction; Acids/bases; Bioanalytical chemistry; Biophysical chemistry; Proteins/peptides; Thermodynamics
• Online Access: https://repository.li.mahidol.ac.th/handle/123456789/3391
• Institution: Mahidol University

The modulation of ligand pKa due to its surrounding environment is a crucial feature that controls many biological phenomena. For example, the shift in the pKa of substrates or catalytic residues at enzyme active sites upon substrate binding often triggers and controls enzymatic reactions. In this w...