Increased intra- and extracellular granzyme expression in patients with tuberculosis

© 2015 Elsevier Ltd. Summary Tuberculosis (TB) is an important cause of morbidity and mortality worldwide. Granzymes (gzms) are proteases mainly found in cytotoxic lymphocytes, but also extracellularly. While the role of gzms in target cell death has been widely characterized, considerable evidence...

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Main Authors: M. Isabel Garcia-Laorden, Dana C. Blok, Liesbeth M. Kager, Arie J. Hoogendijk, Gerard J. Van Mierlo, Ivar O. Lede, Wahid Rahman, Rumana Afroz, Aniruddha Ghose, Caroline E. Visser, Abu Shahed Md Zahed, Md Anwar Husain, Khan Mashrequl Alam, Pravat Chandra Barua, Mahtabuddin Hassan, Ahmed Hossain, Md Abu Tayab, Nick Day, Arjen M. Dondorp, Alex F. De Vos, Tom Van Der Poll
Other Authors: Academic Medical Centre, University of Amsterdam
Format: Article
Published: 2018
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Online Access:https://repository.li.mahidol.ac.th/handle/123456789/36083
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spelling th-mahidol.360832018-11-23T17:37:50Z Increased intra- and extracellular granzyme expression in patients with tuberculosis M. Isabel Garcia-Laorden Dana C. Blok Liesbeth M. Kager Arie J. Hoogendijk Gerard J. Van Mierlo Ivar O. Lede Wahid Rahman Rumana Afroz Aniruddha Ghose Caroline E. Visser Abu Shahed Md Zahed Md Anwar Husain Khan Mashrequl Alam Pravat Chandra Barua Mahtabuddin Hassan Ahmed Hossain Md Abu Tayab Nick Day Arjen M. Dondorp Alex F. De Vos Tom Van Der Poll Academic Medical Centre, University of Amsterdam Sanquin Research Chittagong Medical College Hospital National TB Control Programme Chest Disease Clinic Chittagong (CDCC) Chittagong General Hospital Mahidol University Immunology and Microbiology Medicine © 2015 Elsevier Ltd. Summary Tuberculosis (TB) is an important cause of morbidity and mortality worldwide. Granzymes (gzms) are proteases mainly found in cytotoxic lymphocytes, but also extracellularly. While the role of gzms in target cell death has been widely characterized, considerable evidence points towards broader roles related to infectious and inflammatory responses. To investigate the expression of the gzms in TB, intracellular gzms A, B and K were measured by flow cytometry in lymphocyte populations from peripheral blood mononuclear cells from 18 TB patients and 12 healthy donors from Bangladesh, and extracellular levels of gzmA and B were measured in serum from 58 TB patients and 31 healthy controls. TB patients showed increased expression of gzmA in CD8+ T, CD4+ T and CD56+ T, but not NK, cells, and of gzmB in CD8+ T cells, when compared to controls. GzmK expression was not altered in TB patients in any lymphocyte subset. The extracellular levels of gzmA and, to a lesser extent, of gzmB, were increased in TB patients, but did not correlate with intracellular gzm expression in lymphocyte subsets. Our results reveal enhanced intra- and extracellular expression of gzmA and B in patients with pulmonary TB, suggesting that gzms are part of the host response to tuberculosis. 2018-11-23T10:16:10Z 2018-11-23T10:16:10Z 2015-09-01 Article Tuberculosis. Vol.95, No.5 (2015), 575-580 10.1016/j.tube.2015.05.016 1873281X 14729792 2-s2.0-84940460085 https://repository.li.mahidol.ac.th/handle/123456789/36083 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84940460085&origin=inward
institution Mahidol University
building Mahidol University Library
continent Asia
country Thailand
Thailand
content_provider Mahidol University Library
collection Mahidol University Institutional Repository
topic Immunology and Microbiology
Medicine
spellingShingle Immunology and Microbiology
Medicine
M. Isabel Garcia-Laorden
Dana C. Blok
Liesbeth M. Kager
Arie J. Hoogendijk
Gerard J. Van Mierlo
Ivar O. Lede
Wahid Rahman
Rumana Afroz
Aniruddha Ghose
Caroline E. Visser
Abu Shahed Md Zahed
Md Anwar Husain
Khan Mashrequl Alam
Pravat Chandra Barua
Mahtabuddin Hassan
Ahmed Hossain
Md Abu Tayab
Nick Day
Arjen M. Dondorp
Alex F. De Vos
Tom Van Der Poll
Increased intra- and extracellular granzyme expression in patients with tuberculosis
description © 2015 Elsevier Ltd. Summary Tuberculosis (TB) is an important cause of morbidity and mortality worldwide. Granzymes (gzms) are proteases mainly found in cytotoxic lymphocytes, but also extracellularly. While the role of gzms in target cell death has been widely characterized, considerable evidence points towards broader roles related to infectious and inflammatory responses. To investigate the expression of the gzms in TB, intracellular gzms A, B and K were measured by flow cytometry in lymphocyte populations from peripheral blood mononuclear cells from 18 TB patients and 12 healthy donors from Bangladesh, and extracellular levels of gzmA and B were measured in serum from 58 TB patients and 31 healthy controls. TB patients showed increased expression of gzmA in CD8+ T, CD4+ T and CD56+ T, but not NK, cells, and of gzmB in CD8+ T cells, when compared to controls. GzmK expression was not altered in TB patients in any lymphocyte subset. The extracellular levels of gzmA and, to a lesser extent, of gzmB, were increased in TB patients, but did not correlate with intracellular gzm expression in lymphocyte subsets. Our results reveal enhanced intra- and extracellular expression of gzmA and B in patients with pulmonary TB, suggesting that gzms are part of the host response to tuberculosis.
author2 Academic Medical Centre, University of Amsterdam
author_facet Academic Medical Centre, University of Amsterdam
M. Isabel Garcia-Laorden
Dana C. Blok
Liesbeth M. Kager
Arie J. Hoogendijk
Gerard J. Van Mierlo
Ivar O. Lede
Wahid Rahman
Rumana Afroz
Aniruddha Ghose
Caroline E. Visser
Abu Shahed Md Zahed
Md Anwar Husain
Khan Mashrequl Alam
Pravat Chandra Barua
Mahtabuddin Hassan
Ahmed Hossain
Md Abu Tayab
Nick Day
Arjen M. Dondorp
Alex F. De Vos
Tom Van Der Poll
format Article
author M. Isabel Garcia-Laorden
Dana C. Blok
Liesbeth M. Kager
Arie J. Hoogendijk
Gerard J. Van Mierlo
Ivar O. Lede
Wahid Rahman
Rumana Afroz
Aniruddha Ghose
Caroline E. Visser
Abu Shahed Md Zahed
Md Anwar Husain
Khan Mashrequl Alam
Pravat Chandra Barua
Mahtabuddin Hassan
Ahmed Hossain
Md Abu Tayab
Nick Day
Arjen M. Dondorp
Alex F. De Vos
Tom Van Der Poll
author_sort M. Isabel Garcia-Laorden
title Increased intra- and extracellular granzyme expression in patients with tuberculosis
title_short Increased intra- and extracellular granzyme expression in patients with tuberculosis
title_full Increased intra- and extracellular granzyme expression in patients with tuberculosis
title_fullStr Increased intra- and extracellular granzyme expression in patients with tuberculosis
title_full_unstemmed Increased intra- and extracellular granzyme expression in patients with tuberculosis
title_sort increased intra- and extracellular granzyme expression in patients with tuberculosis
publishDate 2018
url https://repository.li.mahidol.ac.th/handle/123456789/36083
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