High-Resolution Single-Cell Sequencing of Malaria Parasites
© The Author(s) 2017. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution. Single-cell genomics is a powerful tool for determining the genetic architecture of complex communities of unicellular organisms. In areas of high transmission, malaria patients a...
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th-mahidol.413802019-03-14T15:02:20Z High-Resolution Single-Cell Sequencing of Malaria Parasites Simon G. Trevino Standwell C. Nkhoma Shalini Nair Benjamin J. Daniel Karla Moncada Stanley Khoswe Rachel L. Banda François Nosten Ian H. Cheeseman Texas Biomedical Research Institute Malawi-Liverpool-Wellcome Clinical Research Programme Liverpool School of Tropical Medicine Wellcome Trust University of Texas Health Science Center at San Antonio Mahidol University University of Oxford Agricultural and Biological Sciences Biochemistry, Genetics and Molecular Biology © The Author(s) 2017. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution. Single-cell genomics is a powerful tool for determining the genetic architecture of complex communities of unicellular organisms. In areas of high transmission, malaria patients are often challenged by the activities of multiple Plasmodium falciparum lineages, which can potentiate pathology, spread drug resistance loci, and also complicate most genetic analysis. Single-cell sequencing of P. falciparum would be key to understanding infection complexity, though efforts are hampered by the extreme nucleotide composition of its genome (a 1/480% AT-rich). To counter the low coverage achieved in previous studies, we targeted DNA-rich late-stage parasites by Fluorescence-Activated Cell Sorting and whole genome sequencing. Our method routinely generates accurate, near-complete capture of the 23 Mb P. falciparum genome (mean breadth of coverage 90.7%) at high efficiency. Data from 48 single-cell genomes derived from a polyclonal infection sampled in Chikhwawa, Malawi allowed for unambiguous determination of haplotype diversity and recent meiotic events, information that will aid public health efforts. 2018-12-21T06:23:27Z 2019-03-14T08:02:20Z 2018-12-21T06:23:27Z 2019-03-14T08:02:20Z 2017-12-01 Article Genome Biology and Evolution. Vol.9, No.12 (2017), 3373-3383 10.1093/gbe/evx256 17596653 2-s2.0-85042696306 https://repository.li.mahidol.ac.th/handle/123456789/41380 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85042696306&origin=inward |
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Agricultural and Biological Sciences Biochemistry, Genetics and Molecular Biology Simon G. Trevino Standwell C. Nkhoma Shalini Nair Benjamin J. Daniel Karla Moncada Stanley Khoswe Rachel L. Banda François Nosten Ian H. Cheeseman High-Resolution Single-Cell Sequencing of Malaria Parasites |
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© The Author(s) 2017. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution. Single-cell genomics is a powerful tool for determining the genetic architecture of complex communities of unicellular organisms. In areas of high transmission, malaria patients are often challenged by the activities of multiple Plasmodium falciparum lineages, which can potentiate pathology, spread drug resistance loci, and also complicate most genetic analysis. Single-cell sequencing of P. falciparum would be key to understanding infection complexity, though efforts are hampered by the extreme nucleotide composition of its genome (a 1/480% AT-rich). To counter the low coverage achieved in previous studies, we targeted DNA-rich late-stage parasites by Fluorescence-Activated Cell Sorting and whole genome sequencing. Our method routinely generates accurate, near-complete capture of the 23 Mb P. falciparum genome (mean breadth of coverage 90.7%) at high efficiency. Data from 48 single-cell genomes derived from a polyclonal infection sampled in Chikhwawa, Malawi allowed for unambiguous determination of haplotype diversity and recent meiotic events, information that will aid public health efforts. |
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Texas Biomedical Research Institute |
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Texas Biomedical Research Institute Simon G. Trevino Standwell C. Nkhoma Shalini Nair Benjamin J. Daniel Karla Moncada Stanley Khoswe Rachel L. Banda François Nosten Ian H. Cheeseman |
format |
Article |
author |
Simon G. Trevino Standwell C. Nkhoma Shalini Nair Benjamin J. Daniel Karla Moncada Stanley Khoswe Rachel L. Banda François Nosten Ian H. Cheeseman |
author_sort |
Simon G. Trevino |
title |
High-Resolution Single-Cell Sequencing of Malaria Parasites |
title_short |
High-Resolution Single-Cell Sequencing of Malaria Parasites |
title_full |
High-Resolution Single-Cell Sequencing of Malaria Parasites |
title_fullStr |
High-Resolution Single-Cell Sequencing of Malaria Parasites |
title_full_unstemmed |
High-Resolution Single-Cell Sequencing of Malaria Parasites |
title_sort |
high-resolution single-cell sequencing of malaria parasites |
publishDate |
2018 |
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https://repository.li.mahidol.ac.th/handle/123456789/41380 |
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1763488002627076096 |