Seroprevalence of Flavivirus Neutralizing Antibodies in Thailand by High-Throughput Neutralization Assay: Endemic Circulation of Zika Virus before 2012
Thailand is a hyperendemic country for flavivirus infections in Southeast Asia. Although the reporting system for flavivirus surveillance in Thailand is well established, syndromic surveillance tends to underestimate the true epidemiological status of flaviviruses due to the majority of infections b...
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th-mahidol.760742022-08-04T15:48:36Z Seroprevalence of Flavivirus Neutralizing Antibodies in Thailand by High-Throughput Neutralization Assay: Endemic Circulation of Zika Virus before 2012 Atsushi Yamanaka Mami Matsuda Tamaki Okabayashi Pannamthip Pitaksajjakul Pongrama Ramasoota Kyoko Saito Masayoshi Fukasawa Kentaro Hanada Tomokazu Matsuura Masamichi Muramatsu Tatsuo Shioda Ryosuke Suzuki Faculty of Tropical Medicine, Mahidol University The Jikei University School of Medicine National Institute of Infectious Diseases Research Institute for Microbial Diseases University of Miyazaki Biochemistry, Genetics and Molecular Biology Immunology and Microbiology Thailand is a hyperendemic country for flavivirus infections in Southeast Asia. Although the reporting system for flavivirus surveillance in Thailand is well established, syndromic surveillance tends to underestimate the true epidemiological status of flaviviruses due to the majority of infections being asymptomatic. To accurately understand the prevalence of flaviviruses in endemic regions, we performed neutralization tests against multiple flaviviruses using 147 serum samples from healthy donors collected from four distinct regions in Thailand. Single-round infectious particles (SRIP) for six flaviviruses, dengue virus types 1 to 4 (DENV-1 to -4), Japanese encephalitis virus (JEV), and Zika virus (ZIKV), were used as antigens for developing a safe, high-throughput neutralization assay. Titers of neutralizing antibodies (NAbs) against the six flaviviruses revealed that DENV-1 and DENV-2, followed by ZIKV were the predominant circulating flaviviruses in a total of four regions, whereas the prevalence of NAbs against JEV varied among regions. Although the seroprevalence of ZIKV was low relative to that of DENV-1 and DENV-2, the findings strongly suggested that ZIKV has been circulating at a sustained level in Thailand since before 2012. These findings not only demonstrated the application of an SRIPneutralization test in a serological study, but also elucidated the circulation and distribution trends of different flaviviruses in Thailand 2022-08-04T08:06:46Z 2022-08-04T08:06:46Z 2021-08-01 Article mSphere. Vol.6, No.4 (2021), 1-12 10.1128/mSphere.00339-21 23795042 2-s2.0-85115040385 https://repository.li.mahidol.ac.th/handle/123456789/76074 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85115040385&origin=inward |
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Biochemistry, Genetics and Molecular Biology Immunology and Microbiology Atsushi Yamanaka Mami Matsuda Tamaki Okabayashi Pannamthip Pitaksajjakul Pongrama Ramasoota Kyoko Saito Masayoshi Fukasawa Kentaro Hanada Tomokazu Matsuura Masamichi Muramatsu Tatsuo Shioda Ryosuke Suzuki Seroprevalence of Flavivirus Neutralizing Antibodies in Thailand by High-Throughput Neutralization Assay: Endemic Circulation of Zika Virus before 2012 |
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Thailand is a hyperendemic country for flavivirus infections in Southeast Asia. Although the reporting system for flavivirus surveillance in Thailand is well established, syndromic surveillance tends to underestimate the true epidemiological status of flaviviruses due to the majority of infections being asymptomatic. To accurately understand the prevalence of flaviviruses in endemic regions, we performed neutralization tests against multiple flaviviruses using 147 serum samples from healthy donors collected from four distinct regions in Thailand. Single-round infectious particles (SRIP) for six flaviviruses, dengue virus types 1 to 4 (DENV-1 to -4), Japanese encephalitis virus (JEV), and Zika virus (ZIKV), were used as antigens for developing a safe, high-throughput neutralization assay. Titers of neutralizing antibodies (NAbs) against the six flaviviruses revealed that DENV-1 and DENV-2, followed by ZIKV were the predominant circulating flaviviruses in a total of four regions, whereas the prevalence of NAbs against JEV varied among regions. Although the seroprevalence of ZIKV was low relative to that of DENV-1 and DENV-2, the findings strongly suggested that ZIKV has been circulating at a sustained level in Thailand since before 2012. These findings not only demonstrated the application of an SRIPneutralization test in a serological study, but also elucidated the circulation and distribution trends of different flaviviruses in Thailand |
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Faculty of Tropical Medicine, Mahidol University |
author_facet |
Faculty of Tropical Medicine, Mahidol University Atsushi Yamanaka Mami Matsuda Tamaki Okabayashi Pannamthip Pitaksajjakul Pongrama Ramasoota Kyoko Saito Masayoshi Fukasawa Kentaro Hanada Tomokazu Matsuura Masamichi Muramatsu Tatsuo Shioda Ryosuke Suzuki |
format |
Article |
author |
Atsushi Yamanaka Mami Matsuda Tamaki Okabayashi Pannamthip Pitaksajjakul Pongrama Ramasoota Kyoko Saito Masayoshi Fukasawa Kentaro Hanada Tomokazu Matsuura Masamichi Muramatsu Tatsuo Shioda Ryosuke Suzuki |
author_sort |
Atsushi Yamanaka |
title |
Seroprevalence of Flavivirus Neutralizing Antibodies in Thailand by High-Throughput Neutralization Assay: Endemic Circulation of Zika Virus before 2012 |
title_short |
Seroprevalence of Flavivirus Neutralizing Antibodies in Thailand by High-Throughput Neutralization Assay: Endemic Circulation of Zika Virus before 2012 |
title_full |
Seroprevalence of Flavivirus Neutralizing Antibodies in Thailand by High-Throughput Neutralization Assay: Endemic Circulation of Zika Virus before 2012 |
title_fullStr |
Seroprevalence of Flavivirus Neutralizing Antibodies in Thailand by High-Throughput Neutralization Assay: Endemic Circulation of Zika Virus before 2012 |
title_full_unstemmed |
Seroprevalence of Flavivirus Neutralizing Antibodies in Thailand by High-Throughput Neutralization Assay: Endemic Circulation of Zika Virus before 2012 |
title_sort |
seroprevalence of flavivirus neutralizing antibodies in thailand by high-throughput neutralization assay: endemic circulation of zika virus before 2012 |
publishDate |
2022 |
url |
https://repository.li.mahidol.ac.th/handle/123456789/76074 |
_version_ |
1763487664315564032 |