Single-genome sequencing reveals within-host evolution of human malaria parasites
Population genomics of bulk malaria infections is unable to examine intrahost evolution; therefore, most work has focused on the role of recombination in generating genetic variation. We used single-cell sequencing protocol for low-parasitaemia infections to generate 406 near-complete single Plasmod...
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th-mahidol.772072022-08-04T15:47:38Z Single-genome sequencing reveals within-host evolution of human malaria parasites Aliou Dia Catherine Jett Simon G. Trevino Cindy S. Chu Kanlaya Sriprawat Timothy J.C. Anderson François Nosten Ian H. Cheeseman Faculty of Tropical Medicine, Mahidol University Texas Biomedical Research Institute Nuffield Department of Medicine Immunology and Microbiology Population genomics of bulk malaria infections is unable to examine intrahost evolution; therefore, most work has focused on the role of recombination in generating genetic variation. We used single-cell sequencing protocol for low-parasitaemia infections to generate 406 near-complete single Plasmodium vivax genomes from 11 patients sampled during sequential febrile episodes. Parasite genomes contain hundreds of de novo mutations, showing strong signatures of selection, which are enriched in the ApiAP2 family of transcription factors, known targets of adaptation. Comparing 315 P. falciparum single-cell genomes from 15 patients with our P. vivax data, we find broad complementary patterns of de novo mutation at the gene and pathway level, revealing the importance of within-host evolution during malaria infections. 2022-08-04T08:47:38Z 2022-08-04T08:47:38Z 2021-10-13 Article Cell Host and Microbe. Vol.29, No.10 (2021), 1496-1506.e3 10.1016/j.chom.2021.08.009 19346069 19313128 2-s2.0-85116921530 https://repository.li.mahidol.ac.th/handle/123456789/77207 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85116921530&origin=inward |
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Immunology and Microbiology Aliou Dia Catherine Jett Simon G. Trevino Cindy S. Chu Kanlaya Sriprawat Timothy J.C. Anderson François Nosten Ian H. Cheeseman Single-genome sequencing reveals within-host evolution of human malaria parasites |
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Population genomics of bulk malaria infections is unable to examine intrahost evolution; therefore, most work has focused on the role of recombination in generating genetic variation. We used single-cell sequencing protocol for low-parasitaemia infections to generate 406 near-complete single Plasmodium vivax genomes from 11 patients sampled during sequential febrile episodes. Parasite genomes contain hundreds of de novo mutations, showing strong signatures of selection, which are enriched in the ApiAP2 family of transcription factors, known targets of adaptation. Comparing 315 P. falciparum single-cell genomes from 15 patients with our P. vivax data, we find broad complementary patterns of de novo mutation at the gene and pathway level, revealing the importance of within-host evolution during malaria infections. |
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Faculty of Tropical Medicine, Mahidol University |
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Faculty of Tropical Medicine, Mahidol University Aliou Dia Catherine Jett Simon G. Trevino Cindy S. Chu Kanlaya Sriprawat Timothy J.C. Anderson François Nosten Ian H. Cheeseman |
format |
Article |
author |
Aliou Dia Catherine Jett Simon G. Trevino Cindy S. Chu Kanlaya Sriprawat Timothy J.C. Anderson François Nosten Ian H. Cheeseman |
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Aliou Dia |
title |
Single-genome sequencing reveals within-host evolution of human malaria parasites |
title_short |
Single-genome sequencing reveals within-host evolution of human malaria parasites |
title_full |
Single-genome sequencing reveals within-host evolution of human malaria parasites |
title_fullStr |
Single-genome sequencing reveals within-host evolution of human malaria parasites |
title_full_unstemmed |
Single-genome sequencing reveals within-host evolution of human malaria parasites |
title_sort |
single-genome sequencing reveals within-host evolution of human malaria parasites |
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2022 |
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https://repository.li.mahidol.ac.th/handle/123456789/77207 |
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1763498168257871872 |