Anti-hypercholesteraemic and antioxidative activities of camel skin gelatin hydrolysate: effect of enzyme type, enzyme: substrate ratio and time of hydrolysis
Camel skin gelatin hydrolysate (CSGH) was prepared using different proteolytic enzymes [alcalase (A), protease (P) and their combination (AP (1:1))], hydrolysis time (2, 4 and 6 h) and enzyme: substrate (E/S) ratio (1%, 3% and 5%). In general, the degree of hydrolysis (DH) increased with increasing...
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th-mahidol.814382023-05-16T17:40:54Z Anti-hypercholesteraemic and antioxidative activities of camel skin gelatin hydrolysate: effect of enzyme type, enzyme: substrate ratio and time of hydrolysis Fawale O.S. Mahidol University Agricultural and Biological Sciences Camel skin gelatin hydrolysate (CSGH) was prepared using different proteolytic enzymes [alcalase (A), protease (P) and their combination (AP (1:1))], hydrolysis time (2, 4 and 6 h) and enzyme: substrate (E/S) ratio (1%, 3% and 5%). In general, the degree of hydrolysis (DH) increased with increasing hydrolysis time for all enzyme treatments. CSGHs generated with alcalase showed significantly higher lipase (LP) inhibitory activity compared with protease-derived CSGH at all hydrolysis conditions (P < 0.05). Whereas higher cholesterol esterase (CE) inhibitory activity was observed with CSGH produced by protease (P < 0.05). However, when CSGHs were produced using AP, the highest LP and CE inhibitory activity were recorded at 3 and 1% E/S ratio during 2 and 4 h of hydrolysis, respectively (P < 0.05). Antioxidant assays revealed that CSGHs showed improved radical scavenging activities compared with native CSG (P < 0.05). Overall results indicated that CSGHs with higher in vitro anti-hypercholesteraemic and antioxidant activities could be obtained by enzymatic hydrolysis. 2023-05-16T10:40:54Z 2023-05-16T10:40:54Z 2023-04-01 Article International Journal of Food Science and Technology Vol.58 No.4 (2023) , 2151-2160 10.1111/ijfs.16224 13652621 09505423 2-s2.0-85144063530 https://repository.li.mahidol.ac.th/handle/123456789/81438 SCOPUS |
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Agricultural and Biological Sciences Fawale O.S. Anti-hypercholesteraemic and antioxidative activities of camel skin gelatin hydrolysate: effect of enzyme type, enzyme: substrate ratio and time of hydrolysis |
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Camel skin gelatin hydrolysate (CSGH) was prepared using different proteolytic enzymes [alcalase (A), protease (P) and their combination (AP (1:1))], hydrolysis time (2, 4 and 6 h) and enzyme: substrate (E/S) ratio (1%, 3% and 5%). In general, the degree of hydrolysis (DH) increased with increasing hydrolysis time for all enzyme treatments. CSGHs generated with alcalase showed significantly higher lipase (LP) inhibitory activity compared with protease-derived CSGH at all hydrolysis conditions (P < 0.05). Whereas higher cholesterol esterase (CE) inhibitory activity was observed with CSGH produced by protease (P < 0.05). However, when CSGHs were produced using AP, the highest LP and CE inhibitory activity were recorded at 3 and 1% E/S ratio during 2 and 4 h of hydrolysis, respectively (P < 0.05). Antioxidant assays revealed that CSGHs showed improved radical scavenging activities compared with native CSG (P < 0.05). Overall results indicated that CSGHs with higher in vitro anti-hypercholesteraemic and antioxidant activities could be obtained by enzymatic hydrolysis. |
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Fawale O.S. |
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Anti-hypercholesteraemic and antioxidative activities of camel skin gelatin hydrolysate: effect of enzyme type, enzyme: substrate ratio and time of hydrolysis |
title_short |
Anti-hypercholesteraemic and antioxidative activities of camel skin gelatin hydrolysate: effect of enzyme type, enzyme: substrate ratio and time of hydrolysis |
title_full |
Anti-hypercholesteraemic and antioxidative activities of camel skin gelatin hydrolysate: effect of enzyme type, enzyme: substrate ratio and time of hydrolysis |
title_fullStr |
Anti-hypercholesteraemic and antioxidative activities of camel skin gelatin hydrolysate: effect of enzyme type, enzyme: substrate ratio and time of hydrolysis |
title_full_unstemmed |
Anti-hypercholesteraemic and antioxidative activities of camel skin gelatin hydrolysate: effect of enzyme type, enzyme: substrate ratio and time of hydrolysis |
title_sort |
anti-hypercholesteraemic and antioxidative activities of camel skin gelatin hydrolysate: effect of enzyme type, enzyme: substrate ratio and time of hydrolysis |
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2023 |
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https://repository.li.mahidol.ac.th/handle/123456789/81438 |
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