#TITLE_ALTERNATIVE#

• Main Author: PUTERI ALIM PARAMASINTA NIM : 10714095, NINDYA
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/29664
• Institution: Institut Teknologi Bandung

Tea (Camellia sinensis L.) is one of the most frequently consumed beverages because it has <br /> <br /> biological effects. One of tea components is epicatechin, which has biological effect as strong <br /> <br /> antioxidant. High performance liquid chromatography (HPLC), UV Vis spectrophotometry, and TLC <br /> <br /> densitometry have been used for determination of epicatechin in a variety of samples. The <br /> <br /> purpose of this study was to obtain and validate the method of determining epicatechin contents <br /> <br /> in white tea extract using TLC densitometry and determination of epicatechin contents in sample. <br /> <br /> The first step of this study was characterization of crude drug and extract. Further, optimization of <br /> <br /> mobile phase and saturation of chamber were done to obtain optimum condition. The validation <br /> <br /> of methods consist of evaluations on specificity, linearity, limit of detection, limit of <br /> <br /> quantification, accuracy, and presision. Contents of epicatechin were also determined in white tea <br /> <br /> extract sample using validated methods. The best composition of mobile phase were chloroform– <br /> <br /> aseton–formic acid (5:4:1) with 30 minutes for chamber saturation. The values of linearity, <br /> <br /> accuracy recovery value, intraday precision, interday precision, limit of detection, and limit of <br /> <br /> quantification were r = 0.999, r <br /> <br /> 2 <br /> <br /> = 0.998; 90.01%-92.65%; 2.001% and 1.973%; 2.011% and <br /> <br /> 1.934%; 0.537 µg/ spot; 1.628 µg/spot respectively. Based on the methods validation results, <br /> <br /> epicatechin contents in white tea extract sample can be determined using TLC densitometry. The <br /> <br /> average value of epicatechin contents in white tea extract sample was 1398.11 ± 24.61 µg/ mL. <br />