PREPARASI DAN KARAKTERISASI FISIK HOMOGENAT BIJI KENARI (Canarium indicum L.)

• Main Author: Prasetya, Frenky
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/44137
• Institution: Institut Teknologi Bandung

Canaries are plant commodities that grows in eastern parts of Indonesia such as Maluku, North Sulawesi and Seram Island. Canary seeds are nutritious with the largest content of lipid (66.26%) and proteins (13.69%). Canary seeds are often utilized as an additional ingredient of cakes and snacks. This research aims to make a stable homogenized dosage that can be consumed orally to complement human nutritional needs. The nano-sized homogenate is made by the addition of non-ionic surfactant Cremophor RH 40 as emulsifying agent. Stability of homogenate was tested through accelerated stability test at 40 ° C, stability test at room temperature, stability test at 4 °C, Freeze and Thaw stability test and Sentrifuga test. The best stability was obtained at 4 °C with a pH value that did not change significantly from the initial pH of 6.69, no discoloration and odor, and the final particle size of 119 nm. The physical characteristics of the homogenate were specific gravity 1.6709, density 1.6636 g/mL, and viscosity 0.96 cp. The results of the experiment showed that homogenate had a large protein content and some essential fatty acids which are good for the body's nutrition. Total protein levels was tested using the Lowry method. Total protein levels in a blended homogenate before the preparation was 3132.74 ppm. Protein levels after the dialysis processed to eliminate the matrix obtained concentrations of 443.917 ppm with percent recovery during the process of dialysis was 14.17%. In the experiment, there was a reduction in the protein concentration as much as 180.253 ppm of a dosage with an initial pH of 6.69 and a final pH of 4.97 that was stored at room temperature for 3 days. The highest content of fatty acids detected by GC-MS were palmitic acid (53.42%) and oleic acid (43.38%).