AKTIVITAS ANTIOKSIDAN EKSTRAK BIJI WIJEN (Sesamum indicum L.) HITAM DAN PUTIH DENGAN METODE DPPH, FRAP DAN ABTS

• Main Author: Happyniar Trisnawati, Shelvy
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/44372
• Institution: Institut Teknologi Bandung

Antioxidants can scavange free radicals that have free electron on the outer shell and unstable that can attack the other electrons. Free radicals can give some negative effects such as cell function disorders, cell structure damage, degenaratif disease and cancer. Most plants may have antioxidant activity when it contains compounds that can scavange free radicals, such as flavonoids and phenols. Black and white sesame seed that usually used as a food supplement is thought to have antioxidant activity. This research aims to test the antioxidant activity of black and white sesame seed through the determination of IC50 DPPH (2,2-diphenyl-1-picrylhydrazyl), IC50 ABTS (2,2-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid ) and EC50 FRAP (ferric reducing antioxidant power), determining the total phenol and flavonoids, correlations of total phenolic and flavonoid content in each extract with their IC50 of DPPH, IC50 of ABTS, EC50 of FRAP and correlation of three antioxidant methods in sample extracts. Determination of IC50 of DPPH, IC50 of ABTS, EC50 of FRAP, total phenolic, and flavonoid content were performed by UV-visible spectrophotometry and its correlation IC50 of DPPH, IC50 of ABTS, EC50 of FRAP were analyzed by Pearson????s method. Antioxidant activity assays of black and white sesame seed showed that nhexane black sesame seed extract gave the lowest IC50 of DPPH (8,879 µg/mL), ethanol black sesame seed extract gave the lowest IC50 of ABTS (22.907 µg/mL) and ethanol black sesame seed extract gave the lowest EC50 of FRAP (222.401 µg/mL). Ethanol black sesame seed extract gave the higest phenolic content (1.569 g GAE/100 g) and ethyl acetate white sesame seed extract gave the higest flavonoid content (4.290 g QE/100 g). ) Total phenol content in black sesame seed extract had negative and significant correlation with their IC50 of ABTS and EC50 of FRAP, while flavonoid content in white sesame seed extract had negative and significant correlation with their EC50 of FRAP. The results of correlation test between DPPH, ABTS and FRAP methods showed that white sesame seed extract there was positive and significant correlation between IC50 DPPH and EC50 FRAP, while black sesame seed extract gave positive and significant correlation for IC50 ABTS and EC50 FRAP. All seed extracts of black and white sesame were categorized as very strong antioxidant by DPPH methods. Phenolic compounds were major contributor in antioxidant activity of black sesame seed extract by ABTS and FRAP methods, while flavonoid compounds were major contributor in antioxidant activity of white sesame seed extract by FRAP method. DPPH and FRAP methods gave linear results in antioxidant activity of white sesame seed extracts, while ABTS and FRAP methods showed linear result in black sesame seed extract.