Polyphenoloxidase from Starfruit (Averrhoa carambola, L.) I
Polyphenoloxidase (PPO) was z50lated from starfruit (Averrhoa carambola, L.) and purified by acetone precipitation and dialysis. The optimum pH for the aCl}tone purified PPO catalysed oxidation of catechol was found to be pH 7.2 Heat 'inactivation studies showed that the enzyme was heat-labi...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English English |
| Published: |
1986
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| Online Access: | http://psasir.upm.edu.my/id/eprint/2470/1/Polyphenoloxidase_from_Starfruit_%28Averrhoa_carambola%2C_L.%29.pdf http://psasir.upm.edu.my/id/eprint/2470/ |
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| Institution: | Universiti Putra Malaysia |
| Language: | English English |
| Summary: | Polyphenoloxidase (PPO) was z50lated from starfruit (Averrhoa carambola, L.) and purified
by acetone precipitation and dialysis. The optimum pH for the aCl}tone purified PPO catalysed
oxidation of catechol was found to be pH 7.2 Heat 'inactivation studies showed that the enzyme was
heat-labile. Haif-Hvesfor PPO 'inactiivation at 70°C and 75°C were found to be 4.6 min and 3.1 min
respectively. The Michaeline constant (K j and V for catechol with PPO was O. 061M and 20 U ml-1
respectively. |
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