Polyphenoloxidase from Starfruit (Averrhoa carambola, L.) I

Polyphenoloxidase (PPO) was z50lated from starfruit (Averrhoa carambola, L.) and purified by acetone precipitation and dialysis. The optimum pH for the aCl}tone purified PPO catalysed oxidation of catechol was found to be pH 7.2 Heat 'inactivation studies showed that the enzyme was heat-labi...

Full description

Saved in:

Bibliographic Details
Main Authors:	Tengku Adnan, Tengku Adnin, Augustin, M. A., Mohd Ghazali, Hasanah
Format:	Article
Language:	English English
Published:	1986
Online Access:	http://psasir.upm.edu.my/id/eprint/2470/1/Polyphenoloxidase_from_Starfruit_%28Averrhoa_carambola%2C_L.%29.pdf http://psasir.upm.edu.my/id/eprint/2470/
Tags:	Add Tag No Tags, Be the first to tag this record!
Institution:	Universiti Putra Malaysia
Language:	English English

id	my.upm.eprints.2470
record_format	eprints
spelling	my.upm.eprints.24702013-05-27T07:01:18Z http://psasir.upm.edu.my/id/eprint/2470/ Polyphenoloxidase from Starfruit (Averrhoa carambola, L.) I Tengku Adnan, Tengku Adnin Augustin, M. A. Mohd Ghazali, Hasanah Polyphenoloxidase (PPO) was z50lated from starfruit (Averrhoa carambola, L.) and purified by acetone precipitation and dialysis. The optimum pH for the aCl}tone purified PPO catalysed oxidation of catechol was found to be pH 7.2 Heat 'inactivation studies showed that the enzyme was heat-labile. Haif-Hvesfor PPO 'inactiivation at 70°C and 75°C were found to be 4.6 min and 3.1 min respectively. The Michaeline constant (K j and V for catechol with PPO was O. 061M and 20 U ml-1 respectively. 1986 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/2470/1/Polyphenoloxidase_from_Starfruit_%28Averrhoa_carambola%2C_L.%29.pdf Tengku Adnan, Tengku Adnin and Augustin, M. A. and Mohd Ghazali, Hasanah (1986) Polyphenoloxidase from Starfruit (Averrhoa carambola, L.) I. Pertanika, 9 (2). pp. 219-224. English
institution	Universiti Putra Malaysia
building	UPM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Putra Malaysia
content_source	UPM Institutional Repository
url_provider	http://psasir.upm.edu.my/
language	English English
description	Polyphenoloxidase (PPO) was z50lated from starfruit (Averrhoa carambola, L.) and purified by acetone precipitation and dialysis. The optimum pH for the aCl}tone purified PPO catalysed oxidation of catechol was found to be pH 7.2 Heat 'inactivation studies showed that the enzyme was heat-labile. Haif-Hvesfor PPO 'inactiivation at 70°C and 75°C were found to be 4.6 min and 3.1 min respectively. The Michaeline constant (K j and V for catechol with PPO was O. 061M and 20 U ml-1 respectively.
format	Article
author	Tengku Adnan, Tengku Adnin Augustin, M. A. Mohd Ghazali, Hasanah
spellingShingle	Tengku Adnan, Tengku Adnin Augustin, M. A. Mohd Ghazali, Hasanah Polyphenoloxidase from Starfruit (Averrhoa carambola, L.) I
author_facet	Tengku Adnan, Tengku Adnin Augustin, M. A. Mohd Ghazali, Hasanah
author_sort	Tengku Adnan, Tengku Adnin
title	Polyphenoloxidase from Starfruit (Averrhoa carambola, L.) I
title_short	Polyphenoloxidase from Starfruit (Averrhoa carambola, L.) I
title_full	Polyphenoloxidase from Starfruit (Averrhoa carambola, L.) I
title_fullStr	Polyphenoloxidase from Starfruit (Averrhoa carambola, L.) I
title_full_unstemmed	Polyphenoloxidase from Starfruit (Averrhoa carambola, L.) I
title_sort	polyphenoloxidase from starfruit (averrhoa carambola, l.) i
publishDate	1986
url	http://psasir.upm.edu.my/id/eprint/2470/1/Polyphenoloxidase_from_Starfruit_%28Averrhoa_carambola%2C_L.%29.pdf http://psasir.upm.edu.my/id/eprint/2470/
_version_	1643822327980359680

Polyphenoloxidase from Starfruit (Averrhoa carambola, L.) I

Similar Items