Screening of lactic acid bacteria from local dairy products for antimicrobial properties on selected multiple drug resistant clinical isolates

• Main Author: Plantilla, Alfonso Martin E.
• Format: text
• Language: English
• Published: Animo Repository 2015
• Subjects: Dairy products; Lactic acid bacteria; Food Science
• Online Access: https://animorepository.dlsu.edu.ph/etd_bachelors/2792
• Institution: De La Salle University

Probiotic bacteria were isolated from three (3) locally available commercial dairy products namely, Yakult Probiotic Drink, Nestle Cerelac Infant Cereal, and Nido 3+ Powdered Milk. Each probiotic isolate was further examined morphologically and microscopically, and were further characterized using different biochemical tests. The probiotic isolates were identified genotypically through 16S rDNA analysis. The sequences were identified using Basic Local Alignment Search Tool (BLAST). All the probiotic bacterial from the three dairy products were identified to be Lactobacillus casei or Lactobacillus paracasei. The 16S rDNA sequencing results were not able to distinguish between the two species. The probiotic bacteria were established to have antimicrobial activities using the agar overlay method. Test organisms used were the extended-spectrum β-lactamase (ESBL)-producing strains of Klebsiella pneumoniae, and Escherichia coli, metallo-β-lactamase (MBL)-producing strain of Pseudomonas aeruginosa, and a strain of methicillin resistant Staphylococcus aureus (MRSA). The probiotics inhibited the growth of all the tested multiple drug resistant strains.