Bactericidal effects of a new concept of hypochlorous acid water produced by substance-mixing method against typhoidal Salmonella

• Main Authors: Rahman, Habibur, Antonio, Jayson M., Bajgai, Johny, Fadriquela, Ailyn, Sharma, Subham, Trinh, Thuy Thi, Jeong, Yun-Ju, Goh, Seong-Hoon, kim, Cheol-Su, Lee, Kyu-Jae
• Format: text
• Published: Animo Repository 2021
• Subjects: Salmonella infections in animals—Treatment; Acids; Bactericides; Microbiology
• Online Access: https://animorepository.dlsu.edu.ph/faculty_research/11239
• Institution: De La Salle University

Introduction: Typhoidal Salmonella, the causative agent for fowl typhoid and pullorum disease, is the mainspring of high fatalities in poultry breeds. It can be transferred both horizontally within a flock or penetration through the eggshell and vertically from generation to generation via transovarian route. In the event of widespread transmission of Salmonella in fowl and poultry, a rational approach would necessitate prioritizing the use of non-antibiotic agents with bactericidal properties. Hypochlorous acid (HOCI) has received massive attention in research as a new concept of disinfectant due to its high sterilizing properties, easy accessibility, and biosafety. This study was conducted to evaluate the susceptibility of typhoidal Salmonella to HOCI water (Sungjin farm Co. Ltd. Gyeongju-si, Republic of Korea) treatment developed by non-electrolysis and substance-mixing methods. Methods: To confirm the bactericidal effect, the groups were divided into 6: NT (No-treatment; Tryptocase soy broth), TW (tap water) 70% Al (70% ethanol), L-HOCI [27ppm of available chlorine concentration (ACC)], M-HOCI (51ppm of ACC) and H-HOCI (106ppm of ACC) waters. To access effects of typhoidal Salmonella, S. Gallinarum, and S. Pullorum were exposed to these experimental waters. Results. The microbial counts showed that S. Gallinarum and S. Pullorum were more vulnerable to the treatment of 70% ethanol and 3 different concentrations of HOCI water compared to the TW group. Intriguingly, the 70% Al and HOCI groups demonstrated biofilm-inhibiting capabilities toward typhoid Salmonella. Conclusion. Collectively, our study indicates that mixing type of HOCI water could be used as a safe and eco-