Bioethanol production from microalgae Chlorella sorokiniana via simultaneous saccharification and fermentation
This study aimed to investigate the performance of a microalgal feedstock Chlorella sorokiniana in bioethanol production through simultaneous saccharification and fermentation (SSF). The major limiting factor of SSF is the compensation between optimum hydrolysis and fermentation parameters. Using a...
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| Main Authors: | , |
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| Format: | text |
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Animo Repository
2020
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| Online Access: | https://animorepository.dlsu.edu.ph/faculty_research/3710 https://animorepository.dlsu.edu.ph/context/faculty_research/article/4712/type/native/viewcontent/012039.html |
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| Institution: | De La Salle University |
| Summary: | This study aimed to investigate the performance of a microalgal feedstock Chlorella sorokiniana in bioethanol production through simultaneous saccharification and fermentation (SSF). The major limiting factor of SSF is the compensation between optimum hydrolysis and fermentation parameters. Using a central composite design (CCD), three parameters were optimized: biomass concentration, temperature, and pH. For each run, the enzyme mixture (cellulase and amylase) was fixed at 25 g/L, fermentation time at 72 hrs, and inoculum size of Saccharomyces cerevisiae at 20% (v/v). The freeze-dried biomass is simultaneously mixed with the enzyme mixture and the inoculum and placed inside an incubator shaker. The microalgal biomass is found to contain 58.78% total reducing sugars (TRS). Highest ethanol yield obtained is 0.504 g ethanol/g glucose. Using CCD helps navigate the design space and bioprocess. © 2020 IOP Publishing Ltd. |
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