Bioethanol production from microalgae Chlorella sorokiniana via simultaneous saccharification and fermentation

• Main Authors: Tatel, N. J/, Madrazo, C.
• Format: text
• Published: Animo Repository 2020
• Subjects: Chlorella sorokiniana; Biomass energy; Ethanol as fuel; Chemical Engineering
• Online Access: https://animorepository.dlsu.edu.ph/faculty_research/3710; https://animorepository.dlsu.edu.ph/context/faculty_research/article/4712/type/native/viewcontent/012039.html
• Institution: De La Salle University

This study aimed to investigate the performance of a microalgal feedstock Chlorella sorokiniana in bioethanol production through simultaneous saccharification and fermentation (SSF). The major limiting factor of SSF is the compensation between optimum hydrolysis and fermentation parameters. Using a central composite design (CCD), three parameters were optimized: biomass concentration, temperature, and pH. For each run, the enzyme mixture (cellulase and amylase) was fixed at 25 g/L, fermentation time at 72 hrs, and inoculum size of Saccharomyces cerevisiae at 20% (v/v). The freeze-dried biomass is simultaneously mixed with the enzyme mixture and the inoculum and placed inside an incubator shaker. The microalgal biomass is found to contain 58.78% total reducing sugars (TRS). Highest ethanol yield obtained is 0.504 g ethanol/g glucose. Using CCD helps navigate the design space and bioprocess. © 2020 IOP Publishing Ltd.