In vitro expressed GPCR inserted in polymersome membranes for ligand-binding studies

In vitro expressed GPCR inserted in polymersome membranes for ligand-binding studies

The dopamine receptor D2 (DRD2), a G-protein coupled receptor is expressed into PBd22-PEO13 and PMOXA20-PDMS54-PMOXA20 block copolymer vesicles (see scheme). The conformational integrity of the receptor is confirmed by antibody- and ligand-binding assays. Replacement of bound dopamine is demonstrate...

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Bibliographic Details
Main Authors: Low, Ying Xiu, De Hoog, Hans-Peter M., May, Sylvia, Andreasson-Ochsner, Mirjam, Fu, Zhikang, Tan, Darren, Ritz, Sandra, Nallani, Madhavan, Sinner, Eva-Kathrin
Other Authors: School of Materials Science & Engineering
Format: Article
Language:English
Published: 2014
Subjects:
DRNTU::Engineering::Materials::Biomaterials
Online Access:https://hdl.handle.net/10356/102365
http://hdl.handle.net/10220/19016
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Institution: Nanyang Technological University
Language: English
Description
Summary:The dopamine receptor D2 (DRD2), a G-protein coupled receptor is expressed into PBd22-PEO13 and PMOXA20-PDMS54-PMOXA20 block copolymer vesicles (see scheme). The conformational integrity of the receptor is confirmed by antibody- and ligand-binding assays. Replacement of bound dopamine is demonstrated on surface-immobilized polymersomes, thus making this a promising platform for drug screening.

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