In vitro expressed GPCR inserted in polymersome membranes for ligand-binding studies

In vitro expressed GPCR inserted in polymersome membranes for ligand-binding studies

The dopamine receptor D2 (DRD2), a G-protein coupled receptor is expressed into PBd22-PEO13 and PMOXA20-PDMS54-PMOXA20 block copolymer vesicles (see scheme). The conformational integrity of the receptor is confirmed by antibody- and ligand-binding assays. Replacement of bound dopamine is demonstrate...

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Main Authors: Low, Ying Xiu, De Hoog, Hans-Peter M., May, Sylvia, Andreasson-Ochsner, Mirjam, Fu, Zhikang, Tan, Darren, Ritz, Sandra, Nallani, Madhavan, Sinner, Eva-Kathrin
Other Authors: School of Materials Science & Engineering
Format: Article
Language:English
Published: 2014
Subjects:
DRNTU::Engineering::Materials::Biomaterials
Online Access:https://hdl.handle.net/10356/102365
http://hdl.handle.net/10220/19016
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-1023652020-06-01T10:13:45Z In vitro expressed GPCR inserted in polymersome membranes for ligand-binding studies Low, Ying Xiu De Hoog, Hans-Peter M. May, Sylvia Andreasson-Ochsner, Mirjam Fu, Zhikang Tan, Darren Ritz, Sandra Nallani, Madhavan Sinner, Eva-Kathrin School of Materials Science & Engineering DRNTU::Engineering::Materials::Biomaterials The dopamine receptor D2 (DRD2), a G-protein coupled receptor is expressed into PBd22-PEO13 and PMOXA20-PDMS54-PMOXA20 block copolymer vesicles (see scheme). The conformational integrity of the receptor is confirmed by antibody- and ligand-binding assays. Replacement of bound dopamine is demonstrated on surface-immobilized polymersomes, thus making this a promising platform for drug screening. 2014-03-27T09:06:11Z 2019-12-06T20:53:58Z 2014-03-27T09:06:11Z 2019-12-06T20:53:58Z 2013 2013 Journal Article May, S., Andreasson-Ochsner, M., Fu, Z., Low , Y. X., Tan, D., de Hoog, H.-P. M., et al. (2013). In vitro expressed GPCR inserted in polymersome membranes for ligand-binding studies. Angewandte Chemie International Edition, 52(2), 749-753. 1433-7851 https://hdl.handle.net/10356/102365 http://hdl.handle.net/10220/19016 10.1002/anie.201204645 en Angewandte chemie international edition © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
institution Nanyang Technological University
building NTU Library
country Singapore
collection DR-NTU
language English
topic DRNTU::Engineering::Materials::Biomaterials
spellingShingle DRNTU::Engineering::Materials::Biomaterials
Low, Ying Xiu
De Hoog, Hans-Peter M.
May, Sylvia
Andreasson-Ochsner, Mirjam
Fu, Zhikang
Tan, Darren
Ritz, Sandra
Nallani, Madhavan
Sinner, Eva-Kathrin
In vitro expressed GPCR inserted in polymersome membranes for ligand-binding studies
description The dopamine receptor D2 (DRD2), a G-protein coupled receptor is expressed into PBd22-PEO13 and PMOXA20-PDMS54-PMOXA20 block copolymer vesicles (see scheme). The conformational integrity of the receptor is confirmed by antibody- and ligand-binding assays. Replacement of bound dopamine is demonstrated on surface-immobilized polymersomes, thus making this a promising platform for drug screening.
author2 School of Materials Science & Engineering
author_facet School of Materials Science & Engineering
Low, Ying Xiu
De Hoog, Hans-Peter M.
May, Sylvia
Andreasson-Ochsner, Mirjam
Fu, Zhikang
Tan, Darren
Ritz, Sandra
Nallani, Madhavan
Sinner, Eva-Kathrin
format Article
author Low, Ying Xiu
De Hoog, Hans-Peter M.
May, Sylvia
Andreasson-Ochsner, Mirjam
Fu, Zhikang
Tan, Darren
Ritz, Sandra
Nallani, Madhavan
Sinner, Eva-Kathrin
author_sort Low, Ying Xiu
title In vitro expressed GPCR inserted in polymersome membranes for ligand-binding studies
title_short In vitro expressed GPCR inserted in polymersome membranes for ligand-binding studies
title_full In vitro expressed GPCR inserted in polymersome membranes for ligand-binding studies
title_fullStr In vitro expressed GPCR inserted in polymersome membranes for ligand-binding studies
title_full_unstemmed In vitro expressed GPCR inserted in polymersome membranes for ligand-binding studies
title_sort in vitro expressed gpcr inserted in polymersome membranes for ligand-binding studies
publishDate 2014
url https://hdl.handle.net/10356/102365
http://hdl.handle.net/10220/19016
_version_ 1681056537784614912

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