In vitro expressed GPCR inserted in polymersome membranes for ligand-binding studies

• Main Authors: Low, Ying Xiu, De Hoog, Hans-Peter M., May, Sylvia, Andreasson-Ochsner, Mirjam, Fu, Zhikang, Tan, Darren, Ritz, Sandra, Nallani, Madhavan, Sinner, Eva-Kathrin
• Other Authors: School of Materials Science & Engineering
• Format: Article
• Language: English
• Published: 2014
• Subjects: DRNTU::Engineering::Materials::Biomaterials
• Online Access: https://hdl.handle.net/10356/102365; http://hdl.handle.net/10220/19016
• Institution: Nanyang Technological University

The dopamine receptor D2 (DRD2), a G-protein coupled receptor is expressed into PBd22-PEO13 and PMOXA20-PDMS54-PMOXA20 block copolymer vesicles (see scheme). The conformational integrity of the receptor is confirmed by antibody- and ligand-binding assays. Replacement of bound dopamine is demonstrate...