Engineering protein thermostability using a generic activity-independent biophysical screen inside the cell

Protein stability is often a limiting factor in the development of commercial proteins and biopharmaceuticals, as well as for biochemical and structural studies. Unfortunately, identifying stabilizing mutations is not trivial since most are neutral or deleterious. Here we describe a high-throughput...

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Bibliographic Details
Main Authors: Asial, Ignacio, Cheng, Yue Xiang, Engman, Henrik, Dollhopf, Maria, Wu, Binghuang, Nordlund, Pär, Cornvik, Tobias
Other Authors: School of Biological Sciences
Format: Article
Language:English
Published: 2014
Subjects:
Online Access:https://hdl.handle.net/10356/103599
http://hdl.handle.net/10220/19332
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Institution: Nanyang Technological University
Language: English