Flexibility of NS5 Methyltransferase-Polymerase Linker Region Is Essential for Dengue Virus Replication

• Main Authors: Zhao, Yongqian, Soh, Tingjin Sherryl, Chan, Kitti Wing Ki, Fung, Sarah Suet Yin, Swaminathan, Kunchithapadam, Lim, Siew Pheng, Shi, Pei-Yong, Huber, Thomas, Lescar, Julien, Luo, Dahai, Vasudevan, Subhash G.
• Other Authors: Diamond, M. S.
• Format: Article
• Language: English
• Published: 2015
• Online Access: https://hdl.handle.net/10356/103841; http://hdl.handle.net/10220/38795
• Institution: Nanyang Technological University

We examined the function of the conserved Val/Ile residue within the dengue virus NS5 interdomain linker (residues 263 to 272) by site-directed mutagenesis. Gly substitution or Gly/Pro insertion after the conserved residue increased the linker flexibility and created slightly attenuated viruses. In...