Single-molecule mechanical folding and unfolding of RNA hairpins : effects of single A-U to A·C pair substitutions and single proton binding and implications for mRNA structure-induced -1 ribosomal frameshifting

• Main Authors: Yang, Lixia, Zhong, Zhensheng, Tong, Cailing, Jia, Huan, Liu, Yiran, Chen, Gang
• Other Authors: School of Physical and Mathematical Sciences
• Format: Article
• Language: English
• Published: 2019
• Subjects: Mechanical; RNA; Science::Chemistry
• Online Access: https://hdl.handle.net/10356/105583; http://hdl.handle.net/10220/50141
• Institution: Nanyang Technological University

A wobble A·C pair can be protonated at near physiological pH to form a more stable wobble A+·C pair. Here, we constructed an RNA hairpin (rHP) and three mutants with one A-U base pair substituted with an A·C mismatch on the top (near the loop, U22C), middle (U25C), and bottom (U29C) positions of the...