Sungeidines from a non-canonical enediyne biosynthetic pathway

Sungeidines from a non-canonical enediyne biosynthetic pathway

We report the genome-guided discovery of sungeidines, a class of microbial secondary metabolites with unique structural features. Despite evolutionary relationships with dynemicin-type enediynes, the sungeidines are produced by a biosynthetic gene cluster (BGC) that exhibits distinct differences fro...

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Bibliographic Details
Main Authors: Low, Zhen Jie, Ma, Guang-Lei, Tran, Hoa Thi, Zou, Yike, Xiong, Juan, Pang, Limei, Nuryyeva, Selbi, Ye, Hong, Hu, Jin-Feng, Houk, K. N., Liang, Zhao-Xun
Other Authors: School of Biological Sciences
Format: Article
Language:English
Published: 2020
Subjects:
Science::Biological sciences::Microbiology
Science::Biological sciences::Biochemistry
Natural Product
Biosynthesis
Online Access:https://hdl.handle.net/10356/138953
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Institution: Nanyang Technological University
Language: English
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Summary:We report the genome-guided discovery of sungeidines, a class of microbial secondary metabolites with unique structural features. Despite evolutionary relationships with dynemicin-type enediynes, the sungeidines are produced by a biosynthetic gene cluster (BGC) that exhibits distinct differences from known enediyne BGCs. Our studies suggest that the sungeidines are assembled from two octaketide chains that are processed differently than those of the dynemicin-type enediynes. The biosynthesis also involves a unique activating sulfotransferase that promotes a dehydration reaction. The loss of genes, including a putative epoxidase gene, is likely to be the main cause of the divergence of the sungeidine pathway from other canonical enediyne pathways. The findings disclose the surprising evolvability of enediyne pathways and set the stage for characterizing the intriguing enzymatic steps in sungeidine biosynthesis.

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