Sungeidines from a non-canonical enediyne biosynthetic pathway
We report the genome-guided discovery of sungeidines, a class of microbial secondary metabolites with unique structural features. Despite evolutionary relationships with dynemicin-type enediynes, the sungeidines are produced by a biosynthetic gene cluster (BGC) that exhibits distinct differences fro...
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sg-ntu-dr.10356-1389532023-02-28T17:10:13Z Sungeidines from a non-canonical enediyne biosynthetic pathway Low, Zhen Jie Ma, Guang-Lei Tran, Hoa Thi Zou, Yike Xiong, Juan Pang, Limei Nuryyeva, Selbi Ye, Hong Hu, Jin-Feng Houk, K. N. Liang, Zhao-Xun School of Biological Sciences Science::Biological sciences::Microbiology Science::Biological sciences::Biochemistry Natural Product Biosynthesis We report the genome-guided discovery of sungeidines, a class of microbial secondary metabolites with unique structural features. Despite evolutionary relationships with dynemicin-type enediynes, the sungeidines are produced by a biosynthetic gene cluster (BGC) that exhibits distinct differences from known enediyne BGCs. Our studies suggest that the sungeidines are assembled from two octaketide chains that are processed differently than those of the dynemicin-type enediynes. The biosynthesis also involves a unique activating sulfotransferase that promotes a dehydration reaction. The loss of genes, including a putative epoxidase gene, is likely to be the main cause of the divergence of the sungeidine pathway from other canonical enediyne pathways. The findings disclose the surprising evolvability of enediyne pathways and set the stage for characterizing the intriguing enzymatic steps in sungeidine biosynthesis. Submitted/Accepted version 2020-05-14T05:22:41Z 2020-05-14T05:22:41Z 2020 Journal Article Low, Z. J., Ma, G.-L., Tran, H. T., Zou, Y., Xiong, J., Pang, L., . . . Liang, Z.-X. (2020). Sungeidines from a non-canonical enediyne biosynthetic pathway. Journal of the American Chemical Society, 142(4), 1673-1679. doi:10.1021/jacs.9b10086 0002-7863 https://hdl.handle.net/10356/138953 10.1021/jacs.9b10086 31922407 2-s2.0-85078686655 4 142 1673 1679 en NRF-SBP-01 MOE2015-T2-2-026 Journal of the American Chemical Society This document is the Accepted Manuscript version of a Published Work that appeared in final form in Journal of the American Chemical Society, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://doi.org/10.1021/jacs.9b10086 application/pdf |
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Science::Biological sciences::Microbiology Science::Biological sciences::Biochemistry Natural Product Biosynthesis Low, Zhen Jie Ma, Guang-Lei Tran, Hoa Thi Zou, Yike Xiong, Juan Pang, Limei Nuryyeva, Selbi Ye, Hong Hu, Jin-Feng Houk, K. N. Liang, Zhao-Xun Sungeidines from a non-canonical enediyne biosynthetic pathway |
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We report the genome-guided discovery of sungeidines, a class of microbial secondary metabolites with unique structural features. Despite evolutionary relationships with dynemicin-type enediynes, the sungeidines are produced by a biosynthetic gene cluster (BGC) that exhibits distinct differences from known enediyne BGCs. Our studies suggest that the sungeidines are assembled from two octaketide chains that are processed differently than those of the dynemicin-type enediynes. The biosynthesis also involves a unique activating sulfotransferase that promotes a dehydration reaction. The loss of genes, including a putative epoxidase gene, is likely to be the main cause of the divergence of the sungeidine pathway from other canonical enediyne pathways. The findings disclose the surprising evolvability of enediyne pathways and set the stage for characterizing the intriguing enzymatic steps in sungeidine biosynthesis. |
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School of Biological Sciences |
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School of Biological Sciences Low, Zhen Jie Ma, Guang-Lei Tran, Hoa Thi Zou, Yike Xiong, Juan Pang, Limei Nuryyeva, Selbi Ye, Hong Hu, Jin-Feng Houk, K. N. Liang, Zhao-Xun |
format |
Article |
author |
Low, Zhen Jie Ma, Guang-Lei Tran, Hoa Thi Zou, Yike Xiong, Juan Pang, Limei Nuryyeva, Selbi Ye, Hong Hu, Jin-Feng Houk, K. N. Liang, Zhao-Xun |
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Low, Zhen Jie |
title |
Sungeidines from a non-canonical enediyne biosynthetic pathway |
title_short |
Sungeidines from a non-canonical enediyne biosynthetic pathway |
title_full |
Sungeidines from a non-canonical enediyne biosynthetic pathway |
title_fullStr |
Sungeidines from a non-canonical enediyne biosynthetic pathway |
title_full_unstemmed |
Sungeidines from a non-canonical enediyne biosynthetic pathway |
title_sort |
sungeidines from a non-canonical enediyne biosynthetic pathway |
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2020 |
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https://hdl.handle.net/10356/138953 |
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1759854755920740352 |