A minimal sequence for left‐handed G‐quadruplex formation
Recently, we observed the first example of a left‐handed G‐quadruplex structure formed by natural DNA, named Z‐G4 . We analysed the Z‐G4 structure and inspected its primary 28‐nt sequence in order to identify motifs that convey the unique left‐handed twist. Using circular dichroism spectroscopy, NMR...
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sg-ntu-dr.10356-1401402023-02-28T19:46:16Z A minimal sequence for left‐handed G‐quadruplex formation Bakalar, Blaž Heddi, Brahim Schmitt, Emmanuelle Mechulam, Yves Phan, Anh Tuân School of Biological Sciences School of Physical and Mathematical Sciences Science::Chemistry Circular Dichroism DNA Recently, we observed the first example of a left‐handed G‐quadruplex structure formed by natural DNA, named Z‐G4 . We analysed the Z‐G4 structure and inspected its primary 28‐nt sequence in order to identify motifs that convey the unique left‐handed twist. Using circular dichroism spectroscopy, NMR spectroscopy, and X‐ray crystallography, we revealed a minimal sequence motif of 12 nt (GTGGTGGTGGTG) for formation of the left‐handed DNA G‐quadruplex, which is found to be highly abundant in the human genome. A systematic analysis of thymine loop mutations revealed a moderate sequence tolerance, which would further broaden the space of sequences prone to left‐handed G‐quadruplex formation. NRF (Natl Research Foundation, S’pore) MOE (Min. of Education, S’pore) Accepted version 2020-05-27T01:06:48Z 2020-05-27T01:06:48Z 2019 Journal Article Bakalar, B., Heddi, B., Schmitt, E., Mechulam, Y., & Phan, A. T. (2019). A minimal sequence for left‐handed G‐quadruplex formation. Angewandte Chemie International Edition, 58(8), 2331-2335. doi:10.1002/anie.201812628 1433-7851 https://hdl.handle.net/10356/140140 10.1002/anie.201812628 30481397 2-s2.0-85060354828 8 58 2331 2335 en Angewandte Chemie International Edition This is the accepted version of the following article: Bakalar, B., Heddi, B., Schmitt, E., Mechulam, Y., & Phan, A. T. (2019). A minimal sequence for left‐handed G‐quadruplex formation. Angewandte Chemie International Edition, 58(8), 2331-2335. doi:10.1002/anie.201812628, which has been published in final form at https://doi.org/10.1002/anie.201812628. This article may be used for non-commercial purposes in accordance with the Wiley Self-Archiving Policy [https://authorservices.wiley.com/authorresources/Journal-Authors/licensing/self-archiving.html]. application/pdf |
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Science::Chemistry Circular Dichroism DNA Bakalar, Blaž Heddi, Brahim Schmitt, Emmanuelle Mechulam, Yves Phan, Anh Tuân A minimal sequence for left‐handed G‐quadruplex formation |
| description |
Recently, we observed the first example of a left‐handed G‐quadruplex structure formed by natural DNA, named Z‐G4 . We analysed the Z‐G4 structure and inspected its primary 28‐nt sequence in order to identify motifs that convey the unique left‐handed twist. Using circular dichroism spectroscopy, NMR spectroscopy, and X‐ray crystallography, we revealed a minimal sequence motif of 12 nt (GTGGTGGTGGTG) for formation of the left‐handed DNA G‐quadruplex, which is found to be highly abundant in the human genome. A systematic analysis of thymine loop mutations revealed a moderate sequence tolerance, which would further broaden the space of sequences prone to left‐handed G‐quadruplex formation. |
| author2 |
School of Biological Sciences |
| author_facet |
School of Biological Sciences Bakalar, Blaž Heddi, Brahim Schmitt, Emmanuelle Mechulam, Yves Phan, Anh Tuân |
| format |
Article |
| author |
Bakalar, Blaž Heddi, Brahim Schmitt, Emmanuelle Mechulam, Yves Phan, Anh Tuân |
| author_sort |
Bakalar, Blaž |
| title |
A minimal sequence for left‐handed G‐quadruplex formation |
| title_short |
A minimal sequence for left‐handed G‐quadruplex formation |
| title_full |
A minimal sequence for left‐handed G‐quadruplex formation |
| title_fullStr |
A minimal sequence for left‐handed G‐quadruplex formation |
| title_full_unstemmed |
A minimal sequence for left‐handed G‐quadruplex formation |
| title_sort |
minimal sequence for left‐handed g‐quadruplex formation |
| publishDate |
2020 |
| url |
https://hdl.handle.net/10356/140140 |
| _version_ |
1759854974637965312 |