Pulsed SILAM reveals in vivo dynamics of murine brain protein translation
Identification of proteins that are synthesized de novo in response to specific microenvironmental cues is critical for understanding molecular mechanisms that underpin vital physiological processes and pathologies. Here, we report that a brief period of SILAM (Stable Isotope Labeling of Mammals) di...
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sg-ntu-dr.10356-1427862023-02-28T16:56:14Z Pulsed SILAM reveals in vivo dynamics of murine brain protein translation Ng, Ser Sue Park, Jung Eun Meng, Wei Chen, Christopher P. Kalaria, Raj N. McCarthy, Neil E. Sze, Siu Kwan School of Biological Sciences Science::Biological sciences Protein Identification Peptides and Proteins Identification of proteins that are synthesized de novo in response to specific microenvironmental cues is critical for understanding molecular mechanisms that underpin vital physiological processes and pathologies. Here, we report that a brief period of SILAM (Stable Isotope Labeling of Mammals) diet enables the determination of biological functions corresponding to actively translating proteins in the mouse brain. Our results demonstrate that the synapse, dendrite, and myelin sheath are highly active neuronal structures that display rapid protein synthesis, producing key mediators of chemical signaling as well as nutrient sensing, lipid metabolism, and amyloid precursor protein processing/stability. Together, these findings confirm that protein metabolic activity varies significantly between brain functional units in vivo. Our data indicate that pulsed SILAM approaches can unravel complex protein expression dynamics in the murine brain and identify active synthetic pathways and associated functions that are likely impaired in neurodegenerative diseases. MOE (Min. of Education, S’pore) Published version 2020-06-30T07:17:59Z 2020-06-30T07:17:59Z 2020 Journal Article Ng, S. S., Park, J. E., Meng, W., Chen, C. P., Kalaria, R. N., McCarthy, N. E., & Sze, S. K. (2020). Pulsed SILAM reveals in vivo dynamics of murine brain protein translation. ACS Omega, 5(23), 13528-13540. doi:10.1021/acsomega.9b04439 2470-1343 https://hdl.handle.net/10356/142786 10.1021/acsomega.9b04439 32566817 2-s2.0-85085742737 23 5 13528 13540 en ACS Omega © 2020 American Chemical Society. This is an open access article published under an ACS AuthorChoice License, which permits copying and redistribution of the article or any adaptations for non-commercial purposes. application/pdf |
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Science::Biological sciences Protein Identification Peptides and Proteins Ng, Ser Sue Park, Jung Eun Meng, Wei Chen, Christopher P. Kalaria, Raj N. McCarthy, Neil E. Sze, Siu Kwan Pulsed SILAM reveals in vivo dynamics of murine brain protein translation |
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Identification of proteins that are synthesized de novo in response to specific microenvironmental cues is critical for understanding molecular mechanisms that underpin vital physiological processes and pathologies. Here, we report that a brief period of SILAM (Stable Isotope Labeling of Mammals) diet enables the determination of biological functions corresponding to actively translating proteins in the mouse brain. Our results demonstrate that the synapse, dendrite, and myelin sheath are highly active neuronal structures that display rapid protein synthesis, producing key mediators of chemical signaling as well as nutrient sensing, lipid metabolism, and amyloid precursor protein processing/stability. Together, these findings confirm that protein metabolic activity varies significantly between brain functional units in vivo. Our data indicate that pulsed SILAM approaches can unravel complex protein expression dynamics in the murine brain and identify active synthetic pathways and associated functions that are likely impaired in neurodegenerative diseases. |
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School of Biological Sciences |
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School of Biological Sciences Ng, Ser Sue Park, Jung Eun Meng, Wei Chen, Christopher P. Kalaria, Raj N. McCarthy, Neil E. Sze, Siu Kwan |
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Article |
author |
Ng, Ser Sue Park, Jung Eun Meng, Wei Chen, Christopher P. Kalaria, Raj N. McCarthy, Neil E. Sze, Siu Kwan |
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Ng, Ser Sue |
title |
Pulsed SILAM reveals in vivo dynamics of murine brain protein translation |
title_short |
Pulsed SILAM reveals in vivo dynamics of murine brain protein translation |
title_full |
Pulsed SILAM reveals in vivo dynamics of murine brain protein translation |
title_fullStr |
Pulsed SILAM reveals in vivo dynamics of murine brain protein translation |
title_full_unstemmed |
Pulsed SILAM reveals in vivo dynamics of murine brain protein translation |
title_sort |
pulsed silam reveals in vivo dynamics of murine brain protein translation |
publishDate |
2020 |
url |
https://hdl.handle.net/10356/142786 |
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1759856877649264640 |