In vitro biocompatibility of diazirine-grafted biomaterials
Photoactivation of aryl-diazirines is an emerging method of rapid, covalent crosslinking under ambient conditions. These attributes make those compounds candidates for grafting onto inert polymer backbones in order to produce stimuli-sensitive biomaterials. However, no risk assessments are available...
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sg-ntu-dr.10356-1444822023-07-14T16:01:23Z In vitro biocompatibility of diazirine-grafted biomaterials Djordjevic, Ivan Wicaksono, Gautama Solic, Ivan Steele, Terry W. J. School of Materials Science and Engineering Engineering::Materials::Biomaterials Engineering::Bioengineering Diazirine Genotoxicity Photoactivation of aryl-diazirines is an emerging method of rapid, covalent crosslinking under ambient conditions. These attributes make those compounds candidates for grafting onto inert polymer backbones in order to produce stimuli-sensitive biomaterials. However, no risk assessments are available to gauge the toxicity of the leachable components after crosslinking activation. Herein, a stimuli-sensitive biomaterial is formulated from diazirine-grafted polycaprolactone tetrol. Also known as CaproGlu, this biomaterial undergoes UVA-activated crosslinking, with many positive attributes toward bioadhesive applications; hydrophobic, solvent-free, liquid at room temperature, and transitions into a foam biorubber after mild UVA illumination. As a model diazirine-grafted biomaterial, hydrolyzed CaproGlu leachates are evaluated for genotoxicity and skin sensitization, namely, Ames test, direct peptide reactivity, and ARE-Nrf2 luciferase assays. The degradation products of diazirine-mediated crosslinking observe little to no risk of in vitro genotoxicity or skin sensitization. Accepted version 2020-11-06T07:00:01Z 2020-11-06T07:00:01Z 2020 Journal Article Djordjevic, I., Wicaksono, G., Solic, I., & Steele, T. W. J. (2020). In vitro biocompatibility of diazirine-grafted biomaterials. Macromolecular Rapid Communications, 41(21), 2000235-. doi:10.1002/marc.202000235 1022-1336 https://hdl.handle.net/10356/144482 10.1002/marc.202000235 21 41 2000235 en Macromolecular Rapid Communications This is the accepted version of the following article: Djordjevic, I., Wicaksono, G., Solic, I., & Steele, T. W. J. (2020). In vitro biocompatibility of diazirine grafted biomaterials. Macromolecular Rapid Communications, 41(21), 2000235-. doi:10.1002/marc.202000235. This article may be used for non-commercial purposes in accordance with the Wiley Self-Archiving Policy [https://authorservices.wiley.com/authorresources/Journal-Authors/licensing/self-arhiving.html]. application/pdf |
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Engineering::Materials::Biomaterials Engineering::Bioengineering Diazirine Genotoxicity Djordjevic, Ivan Wicaksono, Gautama Solic, Ivan Steele, Terry W. J. In vitro biocompatibility of diazirine-grafted biomaterials |
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Photoactivation of aryl-diazirines is an emerging method of rapid, covalent crosslinking under ambient conditions. These attributes make those compounds candidates for grafting onto inert polymer backbones in order to produce stimuli-sensitive biomaterials. However, no risk assessments are available to gauge the toxicity of the leachable components after crosslinking activation. Herein, a stimuli-sensitive biomaterial is formulated from diazirine-grafted polycaprolactone tetrol. Also known as CaproGlu, this biomaterial undergoes UVA-activated crosslinking, with many positive attributes toward bioadhesive applications; hydrophobic, solvent-free, liquid at room temperature, and transitions into a foam biorubber after mild UVA illumination. As a model diazirine-grafted biomaterial, hydrolyzed CaproGlu leachates are evaluated for genotoxicity and skin sensitization, namely, Ames test, direct peptide reactivity, and ARE-Nrf2 luciferase assays. The degradation products of diazirine-mediated crosslinking observe little to no risk of in vitro genotoxicity or skin sensitization. |
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School of Materials Science and Engineering |
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School of Materials Science and Engineering Djordjevic, Ivan Wicaksono, Gautama Solic, Ivan Steele, Terry W. J. |
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Article |
author |
Djordjevic, Ivan Wicaksono, Gautama Solic, Ivan Steele, Terry W. J. |
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Djordjevic, Ivan |
title |
In vitro biocompatibility of diazirine-grafted biomaterials |
title_short |
In vitro biocompatibility of diazirine-grafted biomaterials |
title_full |
In vitro biocompatibility of diazirine-grafted biomaterials |
title_fullStr |
In vitro biocompatibility of diazirine-grafted biomaterials |
title_full_unstemmed |
In vitro biocompatibility of diazirine-grafted biomaterials |
title_sort |
in vitro biocompatibility of diazirine-grafted biomaterials |
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2020 |
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https://hdl.handle.net/10356/144482 |
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1773551315581927424 |