A novel minimal motif for left-handed G-quadruplex formation

• Main Authors: Das, Poulomi, Winnerdy, Fernaldo Richtia, Maity, Arijit, Mechulam, Yves, Phan, Anh Tuân
• Other Authors: School of Physical and Mathematical Sciences
• Format: Article
• Language: English
• Published: 2021
• Subjects: Science::Chemistry::Biochemistry; Science::Biological sciences; G-quadruplexes; Left Handed
• Online Access: https://hdl.handle.net/10356/150047
• Institution: Nanyang Technological University

A recent study on the left-handed G-quadruplex (LHG4) DNA revealed a 12-nt minimal motif GTGGTGGTGGTG with the ability to independently form an LHG4 and to drive an adjacent sequence to LHG4 formation. Here we have identified a second LHG4-forming motif, GGTGGTGGTGTG, and determined the X-ray crystal structure of an LHG4 involving this motif. Our structural analysis indicated the role of split guanines and single thymine loops in promoting LHG4 formation.