Site-specific protein modifications by an engineered asparaginyl endopeptidase from Viola canadensis

• Main Authors: Chen, Yu, Zhang, Dingpeng, Zhang, Xiaohong, Wang, Zhen, Liu, Chuan-Fa, Tam, James P.
• Other Authors: School of Biological Sciences
• Format: Article
• Language: English
• Published: 2021
• Subjects: Science::Biological sciences; Asparaginyl Endopeptidases; Peptide Asparaginyl Ligases
• Online Access: https://hdl.handle.net/10356/154024
• Institution: Nanyang Technological University

Asparaginyl endopeptidases (AEPs) or legumains are Asn/Asp (Asx)-specific proteases that break peptide bonds, but also function as peptide asparaginyl ligases (PALs) that make peptide bonds. This ligase activity can be used for site-specific protein modifications in biochemical and biotechnological...