Four-layered intramolecular parallel G-quadruplex with non-nucleotide loops: an ultra-stable self-folded DNA nano-scaffold
A four-stranded scaffold of nucleic acids termed G-quadruplex (G4) has found growing applications in nano- and biotechnology. Propeller loops are a hallmark of the most stable intramolecular parallel-stranded G4s. To date, propeller loops have been observed to span only a maximum of three G-tetrad l...
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sg-ntu-dr.10356-1583302023-02-28T20:04:03Z Four-layered intramolecular parallel G-quadruplex with non-nucleotide loops: an ultra-stable self-folded DNA nano-scaffold Devi, Gitali Winnerdy, Fernaldo Richtia Ang, Jason Cheng Yu Lim, Kah Wai Phan, Anh Tuân School of Physical and Mathematical Sciences NTU Institute of Structural Biology Science::Chemistry Science::Biological sciences Science::Physics DNA G-Quadruplex A four-stranded scaffold of nucleic acids termed G-quadruplex (G4) has found growing applications in nano- and biotechnology. Propeller loops are a hallmark of the most stable intramolecular parallel-stranded G4s. To date, propeller loops have been observed to span only a maximum of three G-tetrad layers. Going beyond that would allow creation of more stable scaffolds useful for building robust nanodevices. Here we investigate the formation of propeller loops spanning more than three layers. We showed that native nucleotide sequences are incompatible to-wards this goal, and we identified synthetic non-nucleotide linkers that form propeller loop across four layers. With the established linkers, we constructed a four-layered intramolecular parallel-stranded G4, which exhibited ultra-high thermal stability. Control on loop design would augment the toolbox towards engineering of G4-based nano-scaffolds for diverse applications. National Research Foundation (NRF) Submitted/Accepted version This work was supported by Singapore National Research Foundation Investigatorship (NRF-NRFI2017-09) and Nanyang Technological University grants to A.T.P. 2022-05-19T01:07:45Z 2022-05-19T01:07:45Z 2022 Journal Article Devi, G., Winnerdy, F. R., Ang, J. C. Y., Lim, K. W. & Phan, A. T. (2022). Four-layered intramolecular parallel G-quadruplex with non-nucleotide loops: an ultra-stable self-folded DNA nano-scaffold. ACS Nano, 16, 533-540. https://dx.doi.org/10.1021/acsnano.1c07630 1936-0851 https://hdl.handle.net/10356/158330 10.1021/acsnano.1c07630 16 533 540 en NRF-NRFI2017-09 ACS Nano This document is the Accepted Manuscript version of a Published Work that appeared in final form in ACS Nano, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://doi.org/10.1021/acsnano.1c07630. application/pdf |
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Science::Chemistry Science::Biological sciences Science::Physics DNA G-Quadruplex Devi, Gitali Winnerdy, Fernaldo Richtia Ang, Jason Cheng Yu Lim, Kah Wai Phan, Anh Tuân Four-layered intramolecular parallel G-quadruplex with non-nucleotide loops: an ultra-stable self-folded DNA nano-scaffold |
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A four-stranded scaffold of nucleic acids termed G-quadruplex (G4) has found growing applications in nano- and biotechnology. Propeller loops are a hallmark of the most stable intramolecular parallel-stranded G4s. To date, propeller loops have been observed to span only a maximum of three G-tetrad layers. Going beyond that would allow creation of more stable scaffolds useful for building robust nanodevices. Here we investigate the formation of propeller loops spanning more than three layers. We showed that native nucleotide sequences are incompatible to-wards this goal, and we identified synthetic non-nucleotide linkers that form propeller loop across four layers. With the established linkers, we constructed a four-layered intramolecular parallel-stranded G4, which exhibited ultra-high thermal stability. Control on loop design would augment the toolbox towards engineering of G4-based nano-scaffolds for diverse applications. |
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School of Physical and Mathematical Sciences |
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School of Physical and Mathematical Sciences Devi, Gitali Winnerdy, Fernaldo Richtia Ang, Jason Cheng Yu Lim, Kah Wai Phan, Anh Tuân |
format |
Article |
author |
Devi, Gitali Winnerdy, Fernaldo Richtia Ang, Jason Cheng Yu Lim, Kah Wai Phan, Anh Tuân |
author_sort |
Devi, Gitali |
title |
Four-layered intramolecular parallel G-quadruplex with non-nucleotide loops: an ultra-stable self-folded DNA nano-scaffold |
title_short |
Four-layered intramolecular parallel G-quadruplex with non-nucleotide loops: an ultra-stable self-folded DNA nano-scaffold |
title_full |
Four-layered intramolecular parallel G-quadruplex with non-nucleotide loops: an ultra-stable self-folded DNA nano-scaffold |
title_fullStr |
Four-layered intramolecular parallel G-quadruplex with non-nucleotide loops: an ultra-stable self-folded DNA nano-scaffold |
title_full_unstemmed |
Four-layered intramolecular parallel G-quadruplex with non-nucleotide loops: an ultra-stable self-folded DNA nano-scaffold |
title_sort |
four-layered intramolecular parallel g-quadruplex with non-nucleotide loops: an ultra-stable self-folded dna nano-scaffold |
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2022 |
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https://hdl.handle.net/10356/158330 |
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1759855855852847104 |