Contributions to the studies of asparaginyl peptide ligases and to the design of inhibitors targeting bacterial tRNA methyltransferases

Enzymes constitute very important macromolecular catalysts with a wide range of biological activities. During my thesis work, I have studied two important enzymes using a combination of biochemical and structural biology tools. So my thesis is naturally broken down into two main parts, each part bei...

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Main Author:	Wong, Yee Hwa
Other Authors:	Julien Lescar
Format:	Thesis-Doctor of Philosophy
Language:	English
Published:	Nanyang Technological University 2022
Subjects:	Science::Biological sciences
Online Access:	https://hdl.handle.net/10356/159270
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Institution:	Nanyang Technological University
Language:	English

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spelling	sg-ntu-dr.10356-1592702023-02-28T18:44:38Z Contributions to the studies of asparaginyl peptide ligases and to the design of inhibitors targeting bacterial tRNA methyltransferases Wong, Yee Hwa Julien Lescar School of Biological Sciences Julien@ntu.edu.sg Science::Biological sciences Enzymes constitute very important macromolecular catalysts with a wide range of biological activities. During my thesis work, I have studied two important enzymes using a combination of biochemical and structural biology tools. So my thesis is naturally broken down into two main parts, each part being dedicated to the study of one type of enzyme. The first enzyme is a peptide ligase that belongs to the ligase categories. It is an asparaginyl peptide ligase, which is a very powerful precision conjugation tool with many applications in biochemistry and biotechnology. It recognizes and cleaves the peptide bond that follows an Asx (Asn/Asp) residues at P1 position. The second enzyme is a tRNA methyltransferase, TrmD from Pseudomonas aeruginosa, an attractive drug target to overcome bacterial drug resistance. For each enzyme, I have performed a thorough functional and structural characterization and the results I am presenting here have attractive implications in biotechnology and for drug design. Doctor of Philosophy 2022-06-12T12:05:02Z 2022-06-12T12:05:02Z 2021 Thesis-Doctor of Philosophy Wong, Y. H. (2021). Contributions to the studies of asparaginyl peptide ligases and to the design of inhibitors targeting bacterial tRNA methyltransferases. Doctoral thesis, Nanyang Technological University, Singapore. https://hdl.handle.net/10356/159270 https://hdl.handle.net/10356/159270 en This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License (CC BY-NC 4.0). application/pdf Nanyang Technological University
institution	Nanyang Technological University
building	NTU Library
continent	Asia
country	Singapore Singapore
content_provider	NTU Library
collection	DR-NTU
language	English
topic	Science::Biological sciences
spellingShingle	Science::Biological sciences Wong, Yee Hwa Contributions to the studies of asparaginyl peptide ligases and to the design of inhibitors targeting bacterial tRNA methyltransferases
description	Enzymes constitute very important macromolecular catalysts with a wide range of biological activities. During my thesis work, I have studied two important enzymes using a combination of biochemical and structural biology tools. So my thesis is naturally broken down into two main parts, each part being dedicated to the study of one type of enzyme. The first enzyme is a peptide ligase that belongs to the ligase categories. It is an asparaginyl peptide ligase, which is a very powerful precision conjugation tool with many applications in biochemistry and biotechnology. It recognizes and cleaves the peptide bond that follows an Asx (Asn/Asp) residues at P1 position. The second enzyme is a tRNA methyltransferase, TrmD from Pseudomonas aeruginosa, an attractive drug target to overcome bacterial drug resistance. For each enzyme, I have performed a thorough functional and structural characterization and the results I am presenting here have attractive implications in biotechnology and for drug design.
author2	Julien Lescar
author_facet	Julien Lescar Wong, Yee Hwa
format	Thesis-Doctor of Philosophy
author	Wong, Yee Hwa
author_sort	Wong, Yee Hwa
title	Contributions to the studies of asparaginyl peptide ligases and to the design of inhibitors targeting bacterial tRNA methyltransferases
title_short	Contributions to the studies of asparaginyl peptide ligases and to the design of inhibitors targeting bacterial tRNA methyltransferases
title_full	Contributions to the studies of asparaginyl peptide ligases and to the design of inhibitors targeting bacterial tRNA methyltransferases
title_fullStr	Contributions to the studies of asparaginyl peptide ligases and to the design of inhibitors targeting bacterial tRNA methyltransferases
title_full_unstemmed	Contributions to the studies of asparaginyl peptide ligases and to the design of inhibitors targeting bacterial tRNA methyltransferases
title_sort	contributions to the studies of asparaginyl peptide ligases and to the design of inhibitors targeting bacterial trna methyltransferases
publisher	Nanyang Technological University
publishDate	2022
url	https://hdl.handle.net/10356/159270
_version_	1759856473957990400

Contributions to the studies of asparaginyl peptide ligases and to the design of inhibitors targeting bacterial tRNA methyltransferases

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