A conserved arginine in NS5 binds genomic 3' stem-loop RNA for primer-independent initiation of flavivirus RNA replication

• Main Authors: Wang, Sai, Chan, Kitti Wing Ki, Tan, Min Jie Alvin, Flory, Charlotte, Luo, Dahai, Lescar, Julian, Forwood, Jade K., Vasudevan, Subhash G.
• Other Authors: Lee Kong Chian School of Medicine (LKCMedicine)
• Format: Article
• Language: English
• Published: 2022
• Subjects: Science::Medicine; Science::Biological sciences; De Novo Initiation; Dengue NS5 Protein
• Online Access: https://hdl.handle.net/10356/161299
• Institution: Nanyang Technological University

The commitment to replicate the RNA genome of flaviviruses without a primer involves RNA-protein interactions that have been shown to include the recognition of the stem-loop A (SLA) in the 5' untranslated region (UTR) by the nonstructural protein NS5. We show that DENV2 NS5 arginine 888, locat...