Direct identification of A-to-I editing sites with nanopore native RNA sequencing

• Main Authors: Nguyen, Tram Anh, Heng, Joel Jia Wei, Kaewsapsak, Pornchai, Kok, Louis Eng Piew, Stanojević, Dominik, Liu, Hao, Cardilla, Angelysia, Praditya, Albert, Yi, Zirong, Lin, Mingwan, Aw, Ashley Jong Ghut, Ho, Yin Ying, Peh, Esther Kai Lay, Wang, Yuanming, Zhong, Qixing, Heraud-Farlow, Jacki, Xue, Shifeng, Reversade, Bruno, Walkley, Carl, Ho, Ying Swan, Šikić, Mile, Wan, Yue, Tan, Meng How
• Other Authors: School of Chemical and Biomedical Engineering
• Format: Article
• Language: English
• Published: 2022
• Subjects: Engineering::Bioengineering; Science::Biological sciences; Gene Expression; RNA Editing
• Online Access: https://hdl.handle.net/10356/162250
• Institution: Nanyang Technological University

Inosine is a prevalent RNA modification in animals and is formed when an adenosine is deaminated by the ADAR family of enzymes. Traditionally, inosines are identified indirectly as variants from Illumina RNA-sequencing data because they are interpreted as guanosines by cellular machineries. However,...