Rearrangement of a unique Kv1.3 selectivity filter conformation upon binding of a drug
We report two structures of the human voltage-gated potassium channel (Kv) Kv1.3 in immune cells alone (apo-Kv1.3) and bound to an immunomodulatory drug called dalazatide (dalazatide-Kv1.3). Both the apo-Kv1.3 and dalazatide-Kv1.3 structures are in an activated state based on their depolarized volta...
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sg-ntu-dr.10356-1625712023-02-28T17:13:09Z Rearrangement of a unique Kv1.3 selectivity filter conformation upon binding of a drug Tyagi, Anu Ahmed, Tofayel Jian, Shi Bajaj, Saumya Ong, Seow Theng Goay, Stephanie Shee Min Zhao, Yue Vorobyov, Igor Tian, Changlin Chandy, K. George Bhushan, Shashi School of Biological Sciences Lee Kong Chian School of Medicine (LKCMedicine) Nanyang Institute of Structural Biology Science::Biological sciences Ion Channels Potassium Channels We report two structures of the human voltage-gated potassium channel (Kv) Kv1.3 in immune cells alone (apo-Kv1.3) and bound to an immunomodulatory drug called dalazatide (dalazatide-Kv1.3). Both the apo-Kv1.3 and dalazatide-Kv1.3 structures are in an activated state based on their depolarized voltage sensor and open inner gate. In apo-Kv1.3, the aromatic residue in the signature sequence (Y447) adopts a position that diverges 11 Å from other K+ channels. The outer pore is significantly rearranged, causing widening of the selectivity filter and perturbation of ion binding within the filter. This conformation is stabilized by a network of intrasubunit hydrogen bonds. In dalazatide-Kv1.3, binding of dalazatide to the channel's outer vestibule narrows the selectivity filter, Y447 occupies a position seen in other K+ channels, and this conformation is stabilized by a network of intersubunit hydrogen bonds. These remarkable rearrangements in the selectivity filter underlie Kv1.3's transition into the drug-blocked state. Ministry of Education (MOE) Published version This work was supported by the Ministry of Education (MOE) of Singapore Grants MOE2017-T2-2-089 and MOE2020-T1-002-059 to S. Bhushan; Grant MOE2016-T2-2-032 to K.G.C.; and National Institutes of Health (NIH) Grants R01HL128537, R01HL152681, and OT2OD026580, American Heart Association Award 19CDA34770101, Oracle Cloud for Research, and Pittsburgh Supercomputing Center (PSC) Anton 2 allocation MCB160089P to I.V. 2022-10-31T03:10:52Z 2022-10-31T03:10:52Z 2022 Journal Article Tyagi, A., Ahmed, T., Jian, S., Bajaj, S., Ong, S. T., Goay, S. S. M., Zhao, Y., Vorobyov, I., Tian, C., Chandy, K. G. & Bhushan, S. (2022). Rearrangement of a unique Kv1.3 selectivity filter conformation upon binding of a drug. Proceedings of the National Academy of Sciences of the United States of America, 119(5). https://dx.doi.org/10.1073/pnas.2113536119 0027-8424 https://hdl.handle.net/10356/162571 10.1073/pnas.2113536119 35091471 2-s2.0-85123676995 5 119 en MOE2017-T2-2-089 MOE2020-T1-002-059 MOE2016-T2-2-032 Proceedings of the National Academy of Sciences of the United States of America © The Authors. This article is distributed under Creative Commons Attribution-NonCommercialNoDerivatives License 4.0 (CC BY-NC-ND). application/pdf |
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Science::Biological sciences Ion Channels Potassium Channels Tyagi, Anu Ahmed, Tofayel Jian, Shi Bajaj, Saumya Ong, Seow Theng Goay, Stephanie Shee Min Zhao, Yue Vorobyov, Igor Tian, Changlin Chandy, K. George Bhushan, Shashi Rearrangement of a unique Kv1.3 selectivity filter conformation upon binding of a drug |
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We report two structures of the human voltage-gated potassium channel (Kv) Kv1.3 in immune cells alone (apo-Kv1.3) and bound to an immunomodulatory drug called dalazatide (dalazatide-Kv1.3). Both the apo-Kv1.3 and dalazatide-Kv1.3 structures are in an activated state based on their depolarized voltage sensor and open inner gate. In apo-Kv1.3, the aromatic residue in the signature sequence (Y447) adopts a position that diverges 11 Å from other K+ channels. The outer pore is significantly rearranged, causing widening of the selectivity filter and perturbation of ion binding within the filter. This conformation is stabilized by a network of intrasubunit hydrogen bonds. In dalazatide-Kv1.3, binding of dalazatide to the channel's outer vestibule narrows the selectivity filter, Y447 occupies a position seen in other K+ channels, and this conformation is stabilized by a network of intersubunit hydrogen bonds. These remarkable rearrangements in the selectivity filter underlie Kv1.3's transition into the drug-blocked state. |
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School of Biological Sciences |
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School of Biological Sciences Tyagi, Anu Ahmed, Tofayel Jian, Shi Bajaj, Saumya Ong, Seow Theng Goay, Stephanie Shee Min Zhao, Yue Vorobyov, Igor Tian, Changlin Chandy, K. George Bhushan, Shashi |
format |
Article |
author |
Tyagi, Anu Ahmed, Tofayel Jian, Shi Bajaj, Saumya Ong, Seow Theng Goay, Stephanie Shee Min Zhao, Yue Vorobyov, Igor Tian, Changlin Chandy, K. George Bhushan, Shashi |
author_sort |
Tyagi, Anu |
title |
Rearrangement of a unique Kv1.3 selectivity filter conformation upon binding of a drug |
title_short |
Rearrangement of a unique Kv1.3 selectivity filter conformation upon binding of a drug |
title_full |
Rearrangement of a unique Kv1.3 selectivity filter conformation upon binding of a drug |
title_fullStr |
Rearrangement of a unique Kv1.3 selectivity filter conformation upon binding of a drug |
title_full_unstemmed |
Rearrangement of a unique Kv1.3 selectivity filter conformation upon binding of a drug |
title_sort |
rearrangement of a unique kv1.3 selectivity filter conformation upon binding of a drug |
publishDate |
2022 |
url |
https://hdl.handle.net/10356/162571 |
_version_ |
1759855467450859520 |