Arl15 upregulates the TGFβ family signaling by promoting the assembly of the Smad-complex

• المؤلفون الرئيسيون: Shi, Meng, Tie, Hieng Chiong, Divyanshu, Mahajan, Sun, Xiuping, Zhou, Yan, Boh, Boon Kim, Vardy, Leah A., Lu, Lei
• مؤلفون آخرون: School of Biological Sciences
• التنسيق: مقال
• اللغة: English
• منشور في: 2022
• الموضوعات: Science::Biological sciences; Transactivator Protein; Signal Transduction
• الوصول للمادة أونلاين: https://hdl.handle.net/10356/163669

The hallmark event of the canonical transforming growth factor β (TGFβ) family signaling is the assembly of the Smad-complex, consisting of the common Smad, Smad4, and phos-phorylated receptor-regulated Smads. How the Smad-complex is assembled and regulated is still unclear. Here, we report that active Arl15, an Arf-like small G protein, specifically binds to the MH2 domain of Smad4 and colocalizes with Smad4 at the endolysosome. The binding relieves the auto-inhibition of Smad4, which is imposed by the intramolecular interaction between its MH1 and MH2 domains. Activated Smad4 subsequently interacts with phosphorylated receptor-regulated Smads, forming the Smad-complex. Our observations suggest that Smad4 functions as an effector and a GTPase activating protein (GAP) of Arl15. Assembly of the Smad-complex enhances the GAP activity of Smad4 toward Arl15, therefore dissociating Arl15 before the nuclear translocation of the Smad-complex. Our data further demonstrate that Arl15 positively regulates the TGFβ family signaling.