Engineering an autonomous VH domain to modulate intracellular pathways and to interrogate the eIF4F complex

• Main Authors: Frosi, Yuri, Lin, Yen-Chu, Jiang, Shimin, Ramlan, Siti Radhiah, Hew, Kelly, Engman, Alf Henrik, Pillai, Anil, Yeung, Kit, Cheng, Yue Xiang, Cornvik, Tobias, Nordlund, Par, Goh, Megan, Lama, Dilraj, Gates, Zachary P., Verma, Chandra Shekhar, Thean, Dawn, Lane, David P., Asial, Ignacio, Brown, Christopher J.
• Other Authors: School of Biological Sciences
• Format: Article
• Language: English
• Published: 2023
• Subjects: Engineering; Cell Surface Display Techniques; Eukaryotic Initiation Factor-4E
• Online Access: https://hdl.handle.net/10356/170835
• Institution: Nanyang Technological University

An attractive approach to target intracellular macromolecular interfaces and to model putative drug interactions is to design small high-affinity proteins. Variable domains of the immunoglobulin heavy chain (VH domains) are ideal miniproteins, but their development has been restricted by poor intrac...