Engineering an autonomous VH domain to modulate intracellular pathways and to interrogate the eIF4F complex

• المؤلفون الرئيسيون: Frosi, Yuri, Lin, Yen-Chu, Jiang, Shimin, Ramlan, Siti Radhiah, Hew, Kelly, Engman, Alf Henrik, Pillai, Anil, Yeung, Kit, Cheng, Yue Xiang, Cornvik, Tobias, Nordlund, Par, Goh, Megan, Lama, Dilraj, Gates, Zachary P., Verma, Chandra Shekhar, Thean, Dawn, Lane, David P., Asial, Ignacio, Brown, Christopher J.
• مؤلفون آخرون: School of Biological Sciences
• التنسيق: مقال
• اللغة: English
• منشور في: 2023
• الموضوعات: Engineering; Cell Surface Display Techniques; Eukaryotic Initiation Factor-4E
• الوصول للمادة أونلاين: https://hdl.handle.net/10356/170835
• المؤسسة: Nanyang Technological University

An attractive approach to target intracellular macromolecular interfaces and to model putative drug interactions is to design small high-affinity proteins. Variable domains of the immunoglobulin heavy chain (VH domains) are ideal miniproteins, but their development has been restricted by poor intrac...