Monitoring disassembly and cargo release of phase-separated peptide coacervates with native mass spectrometry

• Main Authors: Cerrato, Carmine P., Leppert, Axel, Sun, Yue, Lane, David P., Arsenian-Henriksson, Marie, Miserez, Ali, Landreh, Michael
• Other Authors: School of Materials Science and Engineering
• Format: Article
• Language: English
• Published: 2023
• Subjects: Engineering::Materials; Native Mass Spectrometry; Peptide Coacervates
• Online Access: https://hdl.handle.net/10356/171059
• Institution: Nanyang Technological University

Engineering liquid-liquid phase separation (LLPS) of proteins and peptides holds great promise for the development of therapeutic carriers with intracellular delivery capability but requires accurate determination of their assembly properties in vitro, usually with fluorescently labeled cargo. Here,...