An optimized cyclophosphamide-treated mouse model of Mycobacterium abscessus pulmonary infection

• Main Authors: Sun, Yan, Zhang, Kaixi, Santos, May Delos, Pee, Carmen J. E., Yang, Yanmeng, Kang, Meiqi, Shin, Sung Jae, Chan-Park, Mary B., Pethe, Kevin
• Other Authors: Lee Kong Chian School of Medicine (LKCMedicine)
• Format: Article
• Language: English
• Published: 2024
• Subjects: Medicine, Health and Life Sciences; Mouse model; Lung infection
• Online Access: https://hdl.handle.net/10356/180529
• Institution: Nanyang Technological University

Mycobacterium abscessus pulmonary infections are increasingly problematic, especially for immunocompromised individuals and those with underlying lung conditions. Currently, there is no reliable standardized treatment, underscoring the need for improved preclinical drug testing. We present a simplified immunosuppressed mouse model using only four injections of cyclophosphamide, which allows for sustained M. abscessus lung burden for up to 16 days. This model proved effective for antibiotic efficacy evaluation, as demonstrated with imipenem or amikacin.