Modulation of thelper polarization by GM-CSF and the dectin-1 ligand, curdlan.
Dendritic cells (DCs) work as antigen presenting cells that help in the polarization of CD4 T cells into effector and regulatory subsets. Pathogen recognition receptors (PRRs) like Toll-like receptors (TLRs) and C-type lectin receptors (CLRs) on DCs recognise pathogen-associated molecular patterns (...
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sg-ntu-dr.10356-452682023-02-28T18:01:42Z Modulation of thelper polarization by GM-CSF and the dectin-1 ligand, curdlan. Loy, Yawen. Ruedl Christiane School of Biological Sciences DRNTU::Science::Biological sciences::Microbiology::Immunology Dendritic cells (DCs) work as antigen presenting cells that help in the polarization of CD4 T cells into effector and regulatory subsets. Pathogen recognition receptors (PRRs) like Toll-like receptors (TLRs) and C-type lectin receptors (CLRs) on DCs recognise pathogen-associated molecular patterns (PAMPS) on pathogens. Activation of DCs by TLRs and CLRs agonists can promote Th1 and Th17 anti-fungal responses. CLR Dectin-1 is a specific receptor for 1, 3-glucans. Its activation can induce cytokines responsible for polarization of Th1 and Th17 cells. Granulocyte macrophage colony stimulating factor (GM-CSF) is shown to enhance expression of dectin-1 in DCs. Thus, this study looks into the effect of GM-CSF and dectin-1 specific agonist-curdlan on their role in modulating differentiation of T helper cells. Various TLR agonists were also used in this study. GM-CSF resulted in the increased production of pro-inflammatory cytokines IL-6, IL-12 and IFN necessary for polarization of Th1 and Th17 cells. GM-CSF was able to promote a higher population of Th1 and Th17 cells to some extent. Curdlan was able to elicit Th1 and Th17 responses however; we found that other TLR agonists, for example CpG B, were stronger promoters of Th1 and Th17 anti-fungal immunity. Bachelor of Science in Biological Sciences 2011-06-10T06:25:58Z 2011-06-10T06:25:58Z 2011 2011 Final Year Project (FYP) http://hdl.handle.net/10356/45268 en Nanyang Technological University 33 p. application/pdf |
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DRNTU::Science::Biological sciences::Microbiology::Immunology Loy, Yawen. Modulation of thelper polarization by GM-CSF and the dectin-1 ligand, curdlan. |
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Dendritic cells (DCs) work as antigen presenting cells that help in the polarization of CD4 T cells into effector and regulatory subsets. Pathogen recognition receptors (PRRs) like Toll-like receptors (TLRs) and C-type lectin receptors (CLRs) on DCs recognise pathogen-associated molecular patterns (PAMPS) on pathogens. Activation of DCs by TLRs and CLRs agonists can promote Th1 and Th17 anti-fungal responses. CLR Dectin-1 is a specific receptor for 1, 3-glucans. Its activation can induce cytokines responsible for polarization of Th1 and Th17 cells. Granulocyte macrophage colony stimulating factor (GM-CSF) is shown to enhance expression of dectin-1 in DCs. Thus, this study looks into the effect of GM-CSF and dectin-1 specific agonist-curdlan on their role in modulating differentiation of T helper cells. Various TLR agonists were also used in this study. GM-CSF resulted in the increased production of pro-inflammatory cytokines IL-6, IL-12 and IFN necessary for polarization of Th1 and Th17 cells. GM-CSF was able to promote a higher population of Th1 and Th17 cells to some extent. Curdlan was able to elicit Th1 and Th17 responses however; we found that other TLR agonists, for example CpG B, were stronger promoters of Th1 and Th17 anti-fungal immunity. |
author2 |
Ruedl Christiane |
author_facet |
Ruedl Christiane Loy, Yawen. |
format |
Final Year Project |
author |
Loy, Yawen. |
author_sort |
Loy, Yawen. |
title |
Modulation of thelper polarization by GM-CSF and the dectin-1 ligand, curdlan. |
title_short |
Modulation of thelper polarization by GM-CSF and the dectin-1 ligand, curdlan. |
title_full |
Modulation of thelper polarization by GM-CSF and the dectin-1 ligand, curdlan. |
title_fullStr |
Modulation of thelper polarization by GM-CSF and the dectin-1 ligand, curdlan. |
title_full_unstemmed |
Modulation of thelper polarization by GM-CSF and the dectin-1 ligand, curdlan. |
title_sort |
modulation of thelper polarization by gm-csf and the dectin-1 ligand, curdlan. |
publishDate |
2011 |
url |
http://hdl.handle.net/10356/45268 |
_version_ |
1759857787897118720 |