Generation of female fibroblast cell lines in which Xist is conditionally and allele-specifically deleted from the inactive X chromosome.

• Main Author: Chen, Beibei.
• Other Authors: School of Biological Sciences
• Format: Final Year Project
• Language: English
• Published: 2012
• Subjects: DRNTU::Science::Biological sciences
• Online Access: http://hdl.handle.net/10356/50882
• Institution: Nanyang Technological University

Dosage compensation is accomplished by X chromosome inactivation (XCI) in female cells of placental mammals. The silencing of either the paternal or the maternal X chromosome ensures the equal expression of X-linked genes in males and females. Xist is a critical gene involved in regulating XCI. The...