Incorporation of modified bases into triplex-forming peptide nucleic acids for the recognition of a C-G pyrimidine-purine inversion site of an RNA duplex
Peptide nucleic acid (PNA), an analogue of DNA, can enhance the recognition of specific sequences in RNA duplexes and may be useful therapeutic reagents. In this study, a PNA monomer (Q) containing a N4-(2-guanidoethyl)-5-methylcytosine base was synthesized and incorporated into 8-mer PNAs. Togethe...
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| Format: | Theses and Dissertations |
| Language: | English |
| Published: |
2015
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| Online Access: | http://hdl.handle.net/10356/65663 |
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| Institution: | Nanyang Technological University |
| Language: | English |
| Summary: | Peptide nucleic acid (PNA), an analogue of DNA, can enhance the recognition of specific sequences in RNA duplexes and may be useful therapeutic reagents. In this study, a PNA monomer (Q) containing a N4-(2-guanidoethyl)-5-methylcytosine base was synthesized and incorporated into 8-mer PNAs. Together with previously reported thio-pseudoisocytosine (L) monomer, the non-denaturing polyacrylamide gel electrophoresis results suggest that the PNA is able to selectively target RNA hairpins containing a pyrimidine-purine (C-G) inversion with high binding affinity. The PNA does not bind to DNA hairpins. Thermal melting experiments indicate that PNA containing Q monomer is not able to bind to single-stranded RNA. |
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