Incorporation of modified bases into triplex-forming peptide nucleic acids for the recognition of a C-G pyrimidine-purine inversion site of an RNA duplex

Peptide nucleic acid (PNA), an analogue of DNA, can enhance the recognition of specific sequences in RNA duplexes and may be useful therapeutic reagents. In this study, a PNA monomer (Q) containing a N4-(2-guanidoethyl)-5-methylcytosine base was synthesized and incorporated into 8-mer PNAs. Togethe...

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Main Author:	Toh, Desiree-Faye Kaixin
Other Authors:	Chen Gang
Format:	Theses and Dissertations
Language:	English
Published:	2015
Subjects:	DRNTU::Science::Chemistry::Biochemistry
Online Access:	http://hdl.handle.net/10356/65663
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Institution:	Nanyang Technological University
Language:	English

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spelling	sg-ntu-dr.10356-656632023-02-28T23:50:16Z Incorporation of modified bases into triplex-forming peptide nucleic acids for the recognition of a C-G pyrimidine-purine inversion site of an RNA duplex Toh, Desiree-Faye Kaixin Chen Gang School of Physical and Mathematical Sciences DRNTU::Science::Chemistry::Biochemistry Peptide nucleic acid (PNA), an analogue of DNA, can enhance the recognition of specific sequences in RNA duplexes and may be useful therapeutic reagents. In this study, a PNA monomer (Q) containing a N4-(2-guanidoethyl)-5-methylcytosine base was synthesized and incorporated into 8-mer PNAs. Together with previously reported thio-pseudoisocytosine (L) monomer, the non-denaturing polyacrylamide gel electrophoresis results suggest that the PNA is able to selectively target RNA hairpins containing a pyrimidine-purine (C-G) inversion with high binding affinity. The PNA does not bind to DNA hairpins. Thermal melting experiments indicate that PNA containing Q monomer is not able to bind to single-stranded RNA. Master of Science 2015-12-03T08:39:06Z 2015-12-03T08:39:06Z 2015 2015 Thesis Toh, D.-F. K. (2015). Incorporation of modified bases into triplex-forming peptide nucleic acids for the recognition of a C-G pyrimidine-purine inversion site of an RNA duplex. Master's thesis, Nanyang Technological University, Singapore. http://hdl.handle.net/10356/65663 en 52 p. application/pdf
institution	Nanyang Technological University
building	NTU Library
continent	Asia
country	Singapore Singapore
content_provider	NTU Library
collection	DR-NTU
language	English
topic	DRNTU::Science::Chemistry::Biochemistry
spellingShingle	DRNTU::Science::Chemistry::Biochemistry Toh, Desiree-Faye Kaixin Incorporation of modified bases into triplex-forming peptide nucleic acids for the recognition of a C-G pyrimidine-purine inversion site of an RNA duplex
description	Peptide nucleic acid (PNA), an analogue of DNA, can enhance the recognition of specific sequences in RNA duplexes and may be useful therapeutic reagents. In this study, a PNA monomer (Q) containing a N4-(2-guanidoethyl)-5-methylcytosine base was synthesized and incorporated into 8-mer PNAs. Together with previously reported thio-pseudoisocytosine (L) monomer, the non-denaturing polyacrylamide gel electrophoresis results suggest that the PNA is able to selectively target RNA hairpins containing a pyrimidine-purine (C-G) inversion with high binding affinity. The PNA does not bind to DNA hairpins. Thermal melting experiments indicate that PNA containing Q monomer is not able to bind to single-stranded RNA.
author2	Chen Gang
author_facet	Chen Gang Toh, Desiree-Faye Kaixin
format	Theses and Dissertations
author	Toh, Desiree-Faye Kaixin
author_sort	Toh, Desiree-Faye Kaixin
title	Incorporation of modified bases into triplex-forming peptide nucleic acids for the recognition of a C-G pyrimidine-purine inversion site of an RNA duplex
title_short	Incorporation of modified bases into triplex-forming peptide nucleic acids for the recognition of a C-G pyrimidine-purine inversion site of an RNA duplex
title_full	Incorporation of modified bases into triplex-forming peptide nucleic acids for the recognition of a C-G pyrimidine-purine inversion site of an RNA duplex
title_fullStr	Incorporation of modified bases into triplex-forming peptide nucleic acids for the recognition of a C-G pyrimidine-purine inversion site of an RNA duplex
title_full_unstemmed	Incorporation of modified bases into triplex-forming peptide nucleic acids for the recognition of a C-G pyrimidine-purine inversion site of an RNA duplex
title_sort	incorporation of modified bases into triplex-forming peptide nucleic acids for the recognition of a c-g pyrimidine-purine inversion site of an rna duplex
publishDate	2015
url	http://hdl.handle.net/10356/65663
_version_	1759856411174502400

Incorporation of modified bases into triplex-forming peptide nucleic acids for the recognition of a C-G pyrimidine-purine inversion site of an RNA duplex

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