Native chemical ligation on aspartic acid and its application towards convergent synthesis of homogeneous N-glycoprotein

Total synthesis of native and functional proteins was almost unachievable back in the early 1990s, but thanks to Prof Kent’s development on native chemical ligation (NCL), chemical protein synthesis has become attainable. The basis of NCL technique is the chemoselective reaction between a peptide th...

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Main Author: Chai, Hua
Other Authors: Liu Chuan Fa
Format: Theses and Dissertations
Language:English
Published: 2016
Subjects:
Online Access:http://hdl.handle.net/10356/66214
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-662142023-02-28T23:48:46Z Native chemical ligation on aspartic acid and its application towards convergent synthesis of homogeneous N-glycoprotein Chai, Hua Liu Chuan Fa Liu Xuewei School of Physical and Mathematical Sciences DRNTU::Science::Chemistry::Analytical chemistry::Proteins Total synthesis of native and functional proteins was almost unachievable back in the early 1990s, but thanks to Prof Kent’s development on native chemical ligation (NCL), chemical protein synthesis has become attainable. The basis of NCL technique is the chemoselective reaction between a peptide thioester with a cysteinyl peptide. The reaction takes place in aqueous buffer and produces a native peptide bond at the cysteinyl site with unprotected peptide fragments. Considering the low natural abundance of cysteine, considerable efforts have been made on extending the scope of native chemical ligation to other proteinogenic amino acids. Our group have been interested in this area for years and have successfully demonstrated a dual native chemical ligation on lysine for the synthesis of linear and branched ubiquitin and diubiquitin proteins. Based on our previous knowledge, we wish to extend the NCL strategy to aspartic acid (Asp) residue, which is the core structure for N-glycopeptides. ​Doctor of Philosophy (SPMS) 2016-03-16T06:59:52Z 2016-03-16T06:59:52Z 2016 Thesis http://hdl.handle.net/10356/66214 en 158 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Science::Chemistry::Analytical chemistry::Proteins
spellingShingle DRNTU::Science::Chemistry::Analytical chemistry::Proteins
Chai, Hua
Native chemical ligation on aspartic acid and its application towards convergent synthesis of homogeneous N-glycoprotein
description Total synthesis of native and functional proteins was almost unachievable back in the early 1990s, but thanks to Prof Kent’s development on native chemical ligation (NCL), chemical protein synthesis has become attainable. The basis of NCL technique is the chemoselective reaction between a peptide thioester with a cysteinyl peptide. The reaction takes place in aqueous buffer and produces a native peptide bond at the cysteinyl site with unprotected peptide fragments. Considering the low natural abundance of cysteine, considerable efforts have been made on extending the scope of native chemical ligation to other proteinogenic amino acids. Our group have been interested in this area for years and have successfully demonstrated a dual native chemical ligation on lysine for the synthesis of linear and branched ubiquitin and diubiquitin proteins. Based on our previous knowledge, we wish to extend the NCL strategy to aspartic acid (Asp) residue, which is the core structure for N-glycopeptides.
author2 Liu Chuan Fa
author_facet Liu Chuan Fa
Chai, Hua
format Theses and Dissertations
author Chai, Hua
author_sort Chai, Hua
title Native chemical ligation on aspartic acid and its application towards convergent synthesis of homogeneous N-glycoprotein
title_short Native chemical ligation on aspartic acid and its application towards convergent synthesis of homogeneous N-glycoprotein
title_full Native chemical ligation on aspartic acid and its application towards convergent synthesis of homogeneous N-glycoprotein
title_fullStr Native chemical ligation on aspartic acid and its application towards convergent synthesis of homogeneous N-glycoprotein
title_full_unstemmed Native chemical ligation on aspartic acid and its application towards convergent synthesis of homogeneous N-glycoprotein
title_sort native chemical ligation on aspartic acid and its application towards convergent synthesis of homogeneous n-glycoprotein
publishDate 2016
url http://hdl.handle.net/10356/66214
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