Tissue macrophage complexity as seen through a novel c-kit(Cre) fate mapping mouse

• Main Author: Sheng, Jianpeng
• Other Authors: Klaus Erik Karjalainen
• Format: Theses and Dissertations
• Language: English
• Published: 2016
• Subjects: DRNTU::Science::Biological sciences
• Online Access: http://hdl.handle.net/10356/66369
• Institution: Nanyang Technological University

In this study, I addressed two main questions: first, how does microglia maintain its population; second, ontogenic origins of other tissue macrophages. In order to study the maintenance of microglia, I utlized two new cell ablation tools in order to deplete microglia: F4/80 and CD45-DTR mouse. I also generated a novel novel c-kifMerCreMer mouse strain that allows me to fate map with a new approach macrophages/myeloid cells in various tissues at different times of ontogeny. I identified the microglia stem cells that maintain the whole population locally. In addition, my results showed that different tissues could have three pools of myeloid cells: F4/80hl macrophages, CD11bhl monocyte/macrophage/eosinophil mixtures and F4/80low neutrophils. All the CDHbhl cell mixtures and neutrophils were derived from adult BM. While four categories of F4/80hl macrophages could be defined based on the ontogenetic origins and maintenance dynamics.