Truncation of Cas9 protein for high performance target-specific genome engineering

• Main Author: Wang, Fei
• Other Authors: Tan Meng How
• Format: Theses and Dissertations
• Language: English
• Published: 2017
• Subjects: DRNTU::Engineering::Bioengineering
• Online Access: http://hdl.handle.net/10356/72369
• Institution: Nanyang Technological University

Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR associated proteins (Cas) system has emerged as a genome engineering tool with the programmable guide RNA sequence for specific gene targeting. However, for lentiviral based in vivo testing and engineering such as adeno-as...